@article{oai:nagasaki-u.repo.nii.ac.jp:00006981, author = {Okita, Kogen and Yamazaki, Hideki and Sakiyama, Kazutaka and Yamane, Hikaru and Niina, Shinya and Takatani, Tomohiro and Arakawa, Osamu and Sakakura, Yoshitaka}, issue = {4}, journal = {Ichthyological Research}, month = {Nov}, note = {Behavioral observation was conducted to test whether olfaction is functional to detect tetrodotoxin (TTX) in tiger puffer Takifugu rubripes using Y-maze. We placed either agarose carrier or one agarose and one agarose containing TTX (200 MU) at each head of the channel of Y-maze. Then three non-toxic hatchery-reared juveniles (body length, 5.6 ± 0.4 cm; n = 18) were released into the Y-maze and pecking behavior to carrier was observed for 3 h. The same procedure was tested for olfactory-ablated juveniles and for juveniles taht received sham operation. Juveniles showed significant selectivity to TTX, except for olfactory-ablated juveniles. These results indicate that pufferfish detects TTX by olfactory organ., Ichthyological Research, 60(4), pp.386-389; 2013}, pages = {386--389}, title = {Puffer smells tetrodotoxin}, volume = {60}, year = {2013} }