@article{oai:nagasaki-u.repo.nii.ac.jp:00007015,
 author = {Kurosaki, Tomoaki and Kodama, Yukinobu and Muro, Takahiro and Higuchi, Norihide and Nakamura, Tadahiro and Kitahara, Takashi and Miyakoda, Mana and Yui, Katsuyuki and Sasaki, Hitoshi},
 issue = {11},
 journal = {Biological and Pharmaceutical Bulletin},
 month = {Nov},
 note = {In this experiment, we developed a novel safe and effective gene delivery vector coated with γ-polyglutamic acid (γ-PGA-coated complexes). The γ-PGA-coated complex was composed of chiseled spherical nano-particles with anionic charges. The plasmid DNA/polyethyleneimine complex (non-coated complex) showed high transgene efficiency in the spleen and lung after intravenous administration in mice, with high liver toxicity and lethality. On the other hand, γ-PGA-coated complex selectively showed high transgene efficiency in the spleen without such toxicity. Furthermore, the γ-PGA-coated complex highly accumulated and showed high gene expression in the marginal zone of the spleen. Those results strongly indicated that γ-PGA-coated complex was suitable as a DNA vaccine vector. We therefore applied γ-PGA-coated complex to melanoma DNA vaccine, pUb-M. The γ-PGA-coated complex containing pUb-M significantly inhibited the growth and metastasis of a melanoma cell line, B16-F10 cells. In conclusion, we developed a splenic gene vector, γ-PGA-coated complex, as a novel technology for clinical vaccination., Biological and Pharmaceutical Bulletin, 36(11), pp.1800-1806; 2013},
 pages = {1800--1806},
 title = {Secure Splenic Delivery of Plasmid DNA and Its Application to DNA Vaccine},
 volume = {36},
 year = {2013}
}