{"created":"2023-05-15T16:34:25.451627+00:00","id":7016,"links":{},"metadata":{"_buckets":{"deposit":"5c156059-a38f-4ec7-9610-44020c926a4d"},"_deposit":{"created_by":2,"id":"7016","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"7016"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00007016","sets":["8:9"]},"author_link":["29727","29725","29719","29723","29724","29717","29722","29726","29720","29721","29718"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-11-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageEnd":"1799","bibliographicPageStart":"1794","bibliographicVolumeNumber":"36","bibliographic_titles":[{"bibliographic_title":"Biological and Pharmaceutical Bulletin"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The purpose of the present study was to investigate the usefulness of the ternary complex with protamine and γ-polyglutamic acid (γ-PGA), which are biodegradable materials for foods and medical products, as a safe gene delivery vector. We formed cationic binary complexes (plasmid DNA (pDNA)/protamine complexes) with high transfection efficiency. The binary complex showed slight toxicity probably related to its total cationic charge. Then, we formed ternary complexes (pDNA/protamine/γ-PGA complexes) by addition of anionic polymer, γ-PGA, and they showed no cytotoxicity. The transfection efficiency of the pDNA/protamine/γ-PGA complexes was as high as that of the pDNA/protamine complexes, although their zeta potentials were different. Inhibition study of the gene expressions in B16-F10 cells suggested that pDNA/protamine complexes were taken up by caveolae-mediated endocytosis and macropinocytosis. On the other hand, pDNA/protamine/ γ-PGA complexes were taken up by clathrin-mediated endocytosis and macropinocytosis. Thus, we succeeded in developing the ternary complex as a safe gene delivery vector with biocompatible materials.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Biological and Pharmaceutical Bulletin, 36(11), pp.1794-1799; 2013","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本薬学会"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1248/bpb.b13-00479","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2013 The Pharmaceutical Society of Japan."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09186158","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"13475215","subitem_source_identifier_type":"ISSN"}]},"item_2_text_62":{"attribute_name":"出版者別言語","attribute_value_mlt":[{"subitem_text_value":"Pharmaceutical Society of Japan"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kanda, Kosuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kodama, Yukinobu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kurosaki, Tomoaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Imamura, Masanobu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakagawa, Hiroo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Muro, Takahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Higuchi, Norihide"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamura, Tadahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kitahara, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Honda, Masayuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sasaki, Hitoshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"BPBul36_1794.pdf","filesize":[{"value":"945.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"BPBul36_1794.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/7016/files/BPBul36_1794.pdf"},"version_id":"68c3a206-4a1f-444c-998c-87370c82bf42"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"γ-polyglutamic acid","subitem_subject_scheme":"Other"},{"subitem_subject":"Gene delivery","subitem_subject_scheme":"Other"},{"subitem_subject":"Nanoparticle","subitem_subject_scheme":"Other"},{"subitem_subject":"Non-viral vector","subitem_subject_scheme":"Other"},{"subitem_subject":"Protamine","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Ternary Complex of Plasmid DNA with Protamine and γ-Polyglutamic Acid for Biocompatible Gene Delivery System","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Ternary Complex of Plasmid DNA with Protamine and γ-Polyglutamic Acid for Biocompatible Gene Delivery System"}]},"item_type_id":"2","owner":"2","path":["9"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-01-09"},"publish_date":"2014-01-09","publish_status":"0","recid":"7016","relation_version_is_last":true,"title":["Ternary Complex of Plasmid DNA with Protamine and γ-Polyglutamic Acid for Biocompatible Gene Delivery System"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T02:33:40.268894+00:00"}