@article{oai:nagasaki-u.repo.nii.ac.jp:00007515,
 author = {Fukuma, Aiko and Yoshikawa, Rokusuke and Miyazawa, Takayuki and Yasuda, Jiro},
 issue = {4},
 journal = {PLoS ONE},
 month = {Apr},
 note = {Endogenous retroviruses (ERVs) are integrated as DNA proviruses in the genomes of all mammalian species. Several ERVs are replication-competent and produced as fully infectious viruses from host cell. Thus, live-attenuated vaccines and biological substances have been prepared using the cell lines which may produce ERV. Indeed, we recently reported that several commercial live-attenuated vaccines for pets were contaminated with the infectious feline endogenous retrovirus, RD-114. In this study, to establish a cell line for vaccine manufacture with reduced risk of ERVs, we generated a cell line stably expressing human tetherin (Teth-CRFK cells). The release of infectious ERV from Teth-CRFK cells was suppressed to undetectable levels, while the production of parvovirus in Teth-CRFK cells was similar to that in parental CRFK cells. These observations suggest that Teth-CRFK cells will be useful as a cell line for the manufacture of live-attenuated vaccines or biological substances with reduced risk of ERV., PLoS ONE, 8(4), e61530; 2013},
 title = {A New Approach to Establish a Cell Line with Reduced Risk of Endogenous Retroviruses},
 volume = {8},
 year = {2013}
}