{"created":"2023-05-15T16:29:51.351803+00:00","id":765,"links":{},"metadata":{"_buckets":{"deposit":"e22c9b57-83cf-446c-8727-29cadb92d849"},"_deposit":{"created_by":2,"id":"765","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"765"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00000765","sets":["10:11"]},"author_link":["3579","3580","3578"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-03-05","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"122054","bibliographicVolumeNumber":"1143","bibliographic_titles":[{"bibliographic_title":"Journal of Chromatography B"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Glyoxylic acid (GA) is the intermediate metabolite in various mammalian metabolic pathways. GA showed high reactivity towards formation of advanced glycation end-products (AGEs); the main cause of pathogenesis and complications of many diseases. The presented study aimed to detect GA in healthy and cardiovascular patients’ (CV) sera; however analysis of GA in biological fluid is a challenge and requires chemical derivatization. Hence, a new, highly sensitive, time saving and reproducible pre-column fluorescence derivatization procedure coupled with high performance liquid chromatography (HPLC) method was developed. The derivatization method was based on reaction of 2-aminobenzenthiol (2-ABT), a fluorogenic reagent, with GA in acidic medium to form highly fluorescent thiazine derivative (290 and 390 nm for excitation and emission wavelengths respectively). The fluorescent derivative was separated within 6 min on a reversed-phase ODS column using an isocratic elution with a mixture of methanol?water (70:30, v/v%). The proposed method parameters were optimized and the method was validated. A good linearity in the concentration range (0.05?5.0 μM) was obtained with detection limit (LOD) of 10 nM (200 fmol/injection), which is more sensitive than several previous methods. Moreover, the recovery results were within the range of 85.0?95.5 % and the intra- and inter-day precision results were ?3.5%. It should be emphasized that this method is the first one for monitoring of GA in CV patients; to investigate its role for diagnosis and monitoring the severity and complications of this disease in clinical laboratory.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Journal of Chromatography B, 1143, art.no.122054; 2020","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V."}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.jchromb.2020.122054","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2020 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"15700232","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ali, Marwa F.B."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kishikawa, Naoya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kuroda, Naotaka"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-03-05"}],"displaytype":"detail","filename":"JChromB1143_122054.pdf","filesize":[{"value":"450.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JChromB1143_122054.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/765/files/JChromB1143_122054.pdf"},"version_id":"99728fc4-ac76-4cd5-a6f5-cb6321b51cca"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Glyoxylic acid","subitem_subject_scheme":"Other"},{"subitem_subject":"2-Aminobenzenthiol","subitem_subject_scheme":"Other"},{"subitem_subject":"High Performance Liquid Chromatography(HPLC)","subitem_subject_scheme":"Other"},{"subitem_subject":"Pre-column fluorescence derivatization","subitem_subject_scheme":"Other"},{"subitem_subject":"Serum analysis","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Development of HPLC method for estimation of glyoxylic acid after pre-column fluorescence derivatization approach based on thiazine derivative formation: A new application in healthy and cardiovascular patients’ sera","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Development of HPLC method for estimation of glyoxylic acid after pre-column fluorescence derivatization approach based on thiazine derivative formation: A new application in healthy and cardiovascular patients’ sera"}]},"item_type_id":"2","owner":"2","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-12-18"},"publish_date":"2020-12-18","publish_status":"0","recid":"765","relation_version_is_last":true,"title":["Development of HPLC method for estimation of glyoxylic acid after pre-column fluorescence derivatization approach based on thiazine derivative formation: A new application in healthy and cardiovascular patients’ sera"],"weko_creator_id":"2","weko_shared_id":2},"updated":"2023-05-15T22:58:27.890654+00:00"}