{"created":"2023-05-15T16:29:51.393580+00:00","id":766,"links":{},"metadata":{"_buckets":{"deposit":"534b1135-3405-4a04-8ddf-8a79fa67796a"},"_deposit":{"created_by":2,"id":"766","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"766"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00000766","sets":["10:11"]},"author_link":["3581","3583","3585","3584","3582"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-04-18","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"112215","bibliographicVolumeNumber":"160","bibliographic_titles":[{"bibliographic_title":"Biosensors and Bioelectronics"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Chemiluminescence-enzyme immunoassays make it possible to measure trace components with high sensitivity and selectivity due to the high specificity of the antigen-antibody reaction and the high sensitivity of chemiluminescence assays. However, using an enzyme-labeled antibody suffers from many problems such as low reproducibility due to the instability of the enzyme and inhibition of antigen-antibody reaction due to its steric effect. Therefore, herein we report an innovative non-enzymatic chemiluminescence immunoassays labeling reagent through using quinone as a signal-generating tag coupled with biotin as a binder, to overcome enzymatic labeling problems. Biotinylated-1,4-naphthoquinone (biotin-NQ) was synthesized and characterized and it could produce long-lasting chemiluminescence upon mixing with dithiothreitol and luminol based on the redox cycle of quinone. Biotin-NQ showed exceptional stability towards different stress factors that may be encountered during performing the immunoassay such as high temperatures, highly acidic and alkaline conditions, and repeated freeze-thaw cycles. On the other hand, all these conditions lead to decreased labeling enzyme reactivity due to possible denaturation of its protein structure. Finally, the measurement of the biotin-labeled antibody was successfully performed using biotin-NQ and avidin. As a result, the antibody could be detected down to 25.7 nM which is 2.5 times sensitive than biotin-HRP chemiluminescence-enzyme immunoassays. Moreover, our method was applied successfully for determination of avidin using immobilized biotinylated antibody and biotin-NQ, which simulates immunoassays. Avidin could be detected down to 23.4 nM with excellent linearity (r = 0.996). Accordingly, our developed reagent, biotin-NQ, could be used as a universal highly stable, cost-effective, and steric free non-enzymatic label for immunoassays.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Biosensors and Bioelectronics, 160, art.no.112215; 2020","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V."}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.bios.2020.112215","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2020 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09565663","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"El-Maghrabey, Mahmoud"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kishikawa, Naoya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Harada, Shiori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohyama, Kaname"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kuroda, Naotaka"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-04-18"}],"displaytype":"detail","filename":"Biosens160_112215.pdf","filesize":[{"value":"998.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Biosens160_112215.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/766/files/Biosens160_112215.pdf"},"version_id":"87768041-cde5-4ba6-b298-d75b9370a9db"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Non-enzymatic sensor","subitem_subject_scheme":"Other"},{"subitem_subject":"Biotin","subitem_subject_scheme":"Other"},{"subitem_subject":"Quinone","subitem_subject_scheme":"Other"},{"subitem_subject":"Chemiluminescence","subitem_subject_scheme":"Other"},{"subitem_subject":"Immunoassay","subitem_subject_scheme":"Other"},{"subitem_subject":"Stability","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Quinone-based antibody labeling reagent for enzyme-free chemiluminescent immunoassays. Application to avidin and biotinylated anti-rabbit IgG labeling","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Quinone-based antibody labeling reagent for enzyme-free chemiluminescent immunoassays. Application to avidin and biotinylated anti-rabbit IgG labeling"}]},"item_type_id":"2","owner":"2","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-12-18"},"publish_date":"2020-12-18","publish_status":"0","recid":"766","relation_version_is_last":true,"title":["Quinone-based antibody labeling reagent for enzyme-free chemiluminescent immunoassays. Application to avidin and biotinylated anti-rabbit IgG labeling"],"weko_creator_id":"2","weko_shared_id":2},"updated":"2023-05-15T22:58:26.091425+00:00"}