@article{oai:nagasaki-u.repo.nii.ac.jp:00008277,
 author = {Takemoto, Yoshihiro and Li, Tao-Sheng and Kubo, Masayuki and Ohshima, Mako and Kurazumi, Hiroshi and Ueda, Kazuhiro and Enoki, Tadahiko and Murata, Tomoaki and Hamano, Kimikazu},
 issue = {11},
 journal = {PLoS ONE},
 month = {Nov},
 note = {Delayed wound healing is a serious clinical problem in patients after surgery. A recent study has demonstrated that bone marrow-derived c-kit-positive (c-kit+) cells play important roles in repairing and regenerating various tissues and organs. To examine the hypothesis that surgical injury induces the mobilization and recruitment of c-kit+ cells to accelerate wound healing. Mice were subjected to a left pneumonectomy. The mobilization of c-kit+ cells was monitored after surgery. Using green fluorescent protein (GFP+) bone marrow-transplanted chimera mice, we investigated further whether the mobilized c-kit+ cells were recruited to effect wound healing in a skin puncture model. The group with left pneumonectomies increased the c-kit+ and CD34+ stem cells in peripheral blood 24 h after surgery. At 3 days after surgery, the skin wound size was observed to be significantly smaller, and the number of bone marrow-derived GFP+ cells and GFP+/c-kit+ cells in the wound tissue was significantly greater in mice that had received pneumonectomies, as compared with those that had received a sham operation. Furthermore, some of these GFP+ cells were positively expressed specific markers of macrophages (F4/80), endothelial cells (CD31), and myofibroblasts (αSMA). The administration of AMD3100, an antagonist of a stromal-cell derived factor (SDF)-1/CXCR4 signaling pathway, reduced the number of GFP+ cells in wound tissue and completely negated the accelerated wound healing. Surgical injury induces the mobilization and recruitment of c-kit+ cells to contribute to wound healing. Regulating c-kit+ cells may provide a new approach that accelerates wound healing after surgery., PLoS ONE, 7(11), e48052; 2012},
 title = {The Mobilization and Recruitment of C-Kit+ Cells Contribute to Wound Healing after Surgery},
 volume = {7},
 year = {2012}
}