@article{oai:nagasaki-u.repo.nii.ac.jp:00008368,
 author = {Wang, Yajun and Jiang, Zedong and Kim, Daekyung and Ueno, Mikinori and Okimura, Takasi and Yamaguchi, Kenichi and Oda, Tatsuya},
 journal = {International Journal of Biological Macromolecules},
 month = {Jan},
 note = {In this study, we investigated the bioactivity of ascophyllan in terms of reactive oxygen species (ROS) generation. In RAW264.7 cells, we found that ascophyllan induced ROS generation in a concentration-dependent manner, but with bell-shaped profile. Immunoblot analysis demonstrated that ascophyllan promoted the translocation of cytosolic subunits (p67phox and p47phox) of NADPH oxidase to the plasma membrane. Among mitogen activated protein (MAP) kinase inhibitors tested, JNK inhibitor showed the most potent inhibitory effect on ascophyllan-induced ROS production. Consistently, significant level of phosphorylated JNK MAP kinase was detected in ascophyllan-treated RAW264.7 cells. Our findings suggest for the first time that ascophyllan can stimulate macrophages to produce ROS through the activations of NADPH oxidase and JNK MAP kinase., International Journal of Biological Macromolecules, 52, pp.164-169; 2013},
 pages = {164--169},
 title = {Stimulatory effect of the sulfated polysaccharide ascophyllan on the respiratory burst in RAW264.7 macrophages},
 volume = {52},
 year = {2013}
}