{"created":"2023-05-15T16:35:28.021491+00:00","id":8368,"links":{},"metadata":{"_buckets":{"deposit":"8f22d256-ed9c-44e2-89df-707693aaee15"},"_deposit":{"created_by":2,"id":"8368","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"8368"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00008368","sets":["50:51"]},"author_link":["34144","34148","34145","34150","34149","34146","34147"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"169","bibliographicPageStart":"164","bibliographicVolumeNumber":"52","bibliographic_titles":[{"bibliographic_title":"International Journal of Biological Macromolecules"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In this study, we investigated the bioactivity of ascophyllan in terms of reactive oxygen species (ROS) generation. In RAW264.7 cells, we found that ascophyllan induced ROS generation in a concentration-dependent manner, but with bell-shaped profile. Immunoblot analysis demonstrated that ascophyllan promoted the translocation of cytosolic subunits (p67phox and p47phox) of NADPH oxidase to the plasma membrane. Among mitogen activated protein (MAP) kinase inhibitors tested, JNK inhibitor showed the most potent inhibitory effect on ascophyllan-induced ROS production. Consistently, significant level of phosphorylated JNK MAP kinase was detected in ascophyllan-treated RAW264.7 cells. Our findings suggest for the first time that ascophyllan can stimulate macrophages to produce ROS through the activations of NADPH oxidase and JNK MAP kinase.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"International Journal of Biological Macromolecules, 52, pp.164-169; 2013","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.ijbiomac.2012.09.008","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2012 Elsevier B.V."},{"subitem_rights":"NOTICE: this is the author’s version of a work that was accepted for publication in International Journal of Biological Macromolecules. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in International Journal of Biological Macromolecules, 52(2013)"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"01418130","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Wang, Yajun"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Jiang, Zedong"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kim, Daekyung"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ueno, Mikinori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okimura, Takasi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamaguchi, Kenichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Oda, Tatsuya"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"IJBM52_164.pdf","filesize":[{"value":"487.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"IJBM52_164.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/8368/files/IJBM52_164.pdf"},"version_id":"3fce6739-1578-46f8-af9d-ac6f7b4388e2"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Ascophyllan","subitem_subject_scheme":"Other"},{"subitem_subject":"Ascophyllum nodosum","subitem_subject_scheme":"Other"},{"subitem_subject":"NADPH oxidase","subitem_subject_scheme":"Other"},{"subitem_subject":"RAW264.7 cells","subitem_subject_scheme":"Other"},{"subitem_subject":"Reactive oxygen species","subitem_subject_scheme":"Other"},{"subitem_subject":"Respiratory burst","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Stimulatory effect of the sulfated polysaccharide ascophyllan on the respiratory burst in RAW264.7 macrophages","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Stimulatory effect of the sulfated polysaccharide ascophyllan on the respiratory burst in RAW264.7 macrophages"}]},"item_type_id":"2","owner":"2","path":["51"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-03-28"},"publish_date":"2013-03-28","publish_status":"0","recid":"8368","relation_version_is_last":true,"title":["Stimulatory effect of the sulfated polysaccharide ascophyllan on the respiratory burst in RAW264.7 macrophages"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T02:06:51.111215+00:00"}