{"created":"2023-05-15T16:29:55.100926+00:00","id":853,"links":{},"metadata":{"_buckets":{"deposit":"3521989a-a8e2-402b-bb3e-74784d9dc8ed"},"_deposit":{"created_by":2,"id":"853","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"853"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00000853","sets":["35:36"]},"author_link":["4032","4030","4029","4026","4027","4028","4031","4033"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-03-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"509","bibliographicPageStart":"497","bibliographicVolumeNumber":"101","bibliographic_titles":[{"bibliographic_title":"Journal of General Virology"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Tick-borne encephalitis virus (TBEV) is a zoonotic virus in the genus Flavivirus, family Flaviviridae. TBEV is widely distributed in northern regions of the Eurasian continent, including Japan, and causes severe encephalitis in humans. Tick-borne encephalitis (TBE) was recently reported in central Hokkaido, and wild animals with anti-TBEV antibodies were detected over a wide area of Hokkaido, although TBEV was only isolated in southern Hokkaido. In this study, we conducted a survey of ticks to isolate TBEV in central Hokkaido. One strain, designated Sapporo-17-Io1, was isolated from ticks (Ixodes ovatus) collected in Sapporo city. Sequence analysis revealed that the isolated strain belonged to the Far Eastern subtype of TBEV and was classified in a different subcluster from Oshima 5-10, which had previously been isolated in southern Hokkaido. Sapporo-17-Io1 showed similar growth properties to those of Oshima 5-10 in cultured cells and mouse brains. The mortality rate of mice infected intracerebrally with each virus was similar, but the survival time of mice inoculated with Sapporo-17-Io1 was significantly longer than that of mice inoculated with Oshima 5-10. These results indicate that the neurovirulence of Sapporo-17-Io1 was lower than that of Oshima 5-10. Using an infectious cDNA clone, the replacement of genes encoding non-structural genes from Oshima 5-10 with those from Sapporo-17-Io1 attenuated the neuropathogenicity of the cloned viruses. This result indicated that the non-structural proteins determine the neurovirulence of these two strains. Our results provide important insights for evaluating epidemiological risk in TBE-endemic areas of Hokkaido.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Journal of general virology, 101(5), pp.497-509; 2020","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Microbiology Society"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1099/jgv.0.001400","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2020 The Authors. The definitive peer reviewed, edited version of this article is published in Journal of General Virology, Volume 101, Issue 5, 2020, https://doi.org/10.1099/jgv.0.001400."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00221317","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"14652099","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takahashi, Yuji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kobayashi, Shintaro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ishizuka, Mariko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hirano, Minato"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Muto, Memi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nishiyama, Shoko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kariwa, Hiroaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshii, Kentaro"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-18"}],"displaytype":"detail","filename":"JGV101_497.pdf","filesize":[{"value":"948.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JGV101_497.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/853/files/JGV101_497.pdf"},"version_id":"b9d89526-43da-44a0-ac91-9b69e434f373"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"epidemiology","subitem_subject_scheme":"Other"},{"subitem_subject":"flavivirus","subitem_subject_scheme":"Other"},{"subitem_subject":"neuropathogenicity","subitem_subject_scheme":"Other"},{"subitem_subject":"tick-borne encephalitis virus","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Characterization of tick-borne encephalitis virus isolated from a tick in central Hokkaido in 2017","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Characterization of tick-borne encephalitis virus isolated from a tick in central Hokkaido in 2017"}]},"item_type_id":"2","owner":"2","path":["36"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-06-15"},"publish_date":"2020-06-15","publish_status":"0","recid":"853","relation_version_is_last":true,"title":["Characterization of tick-borne encephalitis virus isolated from a tick in central Hokkaido in 2017"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T04:12:15.397768+00:00"}