{"created":"2023-05-15T16:35:41.859624+00:00","id":8666,"links":{},"metadata":{"_buckets":{"deposit":"5b1df953-2451-41bc-aefc-2c8e58a88105"},"_deposit":{"created_by":2,"id":"8666","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"8666"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00008666","sets":["8:9"]},"author_link":["35064","35058","35066","35060","35059","35065","35061","35062","35063"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"101","bibliographicPageStart":"96","bibliographicVolumeNumber":"36","bibliographic_titles":[{"bibliographic_title":"Biological and Pharmaceutical Bulletin"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In this experiment, we developed anionic ternary complexes for effective and safe ocular gene delivery. Ternary complexes were constructed by coating plasmid DNA (pDNA)/polyethylenimine (PEI) complex with anionic polymers such as γ-polyglutamic acid (γ-PGA) and chondroitin sulfate (CS). The cationic pDNA/PEI complex showed high gene expression on the human retinal pigment epithelial cell line, ARPE-19 cells. The pDNA/PEI complexes, however, also showed high cytotoxicity on the cells and aggregated strongly in the vitreous body. On the other hand, the anionic ternary complexes showed high gene expression on ARPE-19 cells without such cytotoxicity and aggregation. After intravitreous administration of the complexes, the anionic ternary complexes showed high gene expression in the retina. These results strongly indicate that anionic ternary complexes are suitable for effective and safe ocular gene therapy.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Biological and Pharmaceutical Bulletin, 36(1), pp.96-101; 2013","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本薬学会"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1248/bpb.b12-00728","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2013 The Pharmaceutical Society of Japan"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09186158","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"13475215","subitem_source_identifier_type":"ISSN"}]},"item_2_text_62":{"attribute_name":"出版者別言語","attribute_value_mlt":[{"subitem_text_value":"Pharmaceutical Society of Japan"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kurosaki, Tomoaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Uematsu, Masafumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shimoda, Kenichiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuma, Kiyoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakai, Masato"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamura, Tadahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kitahara, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kitaoka, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sasaki, Hitoshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"BPBul36_96.pdf","filesize":[{"value":"2.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"BPBul36_96.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/8666/files/BPBul36_96.pdf"},"version_id":"70c25634-81f4-46ce-a7fb-e990199bd3d5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Biomaterial","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA","subitem_subject_scheme":"Other"},{"subitem_subject":"Gene vector","subitem_subject_scheme":"Other"},{"subitem_subject":"Nanoparticle","subitem_subject_scheme":"Other"},{"subitem_subject":"Non-viral gene delivery","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Ocular Gene Delivery Systems Using Ternary Complexes of Plasmid DNA, Polyethylenimine, and Anionic Polymers","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Ocular Gene Delivery Systems Using Ternary Complexes of Plasmid DNA, Polyethylenimine, and Anionic Polymers"}]},"item_type_id":"2","owner":"2","path":["9"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-04-22"},"publish_date":"2013-04-22","publish_status":"0","recid":"8666","relation_version_is_last":true,"title":["Ocular Gene Delivery Systems Using Ternary Complexes of Plasmid DNA, Polyethylenimine, and Anionic Polymers"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T02:01:09.667428+00:00"}