{"created":"2023-05-15T16:29:56.423410+00:00","id":884,"links":{},"metadata":{"_buckets":{"deposit":"9745543b-24a4-4f3f-a534-7e2bed186df9"},"_deposit":{"created_by":2,"id":"884","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"884"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00000884","sets":["35:36"]},"author_link":["4211","4214","4215","4213","4210","4212","4216"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-06-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageStart":"297","bibliographicVolumeNumber":"8","bibliographic_titles":[{"bibliographic_title":"Vaccines"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Mosquito-borne flavivirus infections, including dengue virus and Zika virus, are major public health threats globally. While the plaque reduction neutralization test (PRNT) is considered the gold standard for determining neutralizing antibody levels to flaviviruses, the assay is time-consuming and laborious. This study, therefore, aimed to develop an enzyme-linked immunosorbent assay (ELISA)-based microneutralization test (EMNT) for the detection of neutralizing antibodies to mosquito-borne flaviviruses. The inhibition of viral growth due to neutralizing antibodies was determined colorimetrically by using EMNT. Given the significance of Fcγ-receptors (FcγR) in antibody-mediated neutralization and antibody-dependent enhancement (ADE) of flavivirus infection, non-FcγR and FcγR-expressing cell lines were used in the EMNT to allow the detection of the sum of neutralizing and immune-enhancing antibody activity as the neutralizing titer. Using anti-flavivirus monoclonal antibodies and clinical samples, the utility of EMNT was evaluated by comparing the end-point titers of the EMNT and the PRNT. The correlation between EMNT and PRNT titers was strong, indicating that EMNT was robust and reproducible. The new EMNT assay combines the biological functional assessment of virus neutralization activity and the technical advantages of ELISA and, is simple, reliable, practical, and could be automated for high-throughput implementation in flavivirus surveillance studies and vaccine trials.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Vaccines, 8(2), art.no.297, pp.1-15; 2020","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/vaccines8020297","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)."}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"2076393X","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Balingit, Jean Claude"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Phu, Ly  Minh Huong"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsuda, Mami"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuki, Ryosuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hasebe, Futoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Morita, Kouichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Moi, Meng Ling"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-18"}],"displaytype":"detail","filename":"VACCINES8_297_.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"VACCINES8_297_.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/884/files/VACCINES8_297_.pdf"},"version_id":"9e863853-b34b-44c8-9173-528aec5d3e6a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Dengue","subitem_subject_scheme":"Other"},{"subitem_subject":"ELISA","subitem_subject_scheme":"Other"},{"subitem_subject":"Flavivirus","subitem_subject_scheme":"Other"},{"subitem_subject":"Microneutralization test","subitem_subject_scheme":"Other"},{"subitem_subject":"Neutralizing antibodies","subitem_subject_scheme":"Other"},{"subitem_subject":"Zika","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A Simple and High-Throughput ELISA-Based Neutralization Assay for the Determination of Anti-Flavivirus Neutralizing Antibodies","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A Simple and High-Throughput ELISA-Based Neutralization Assay for the Determination of Anti-Flavivirus Neutralizing Antibodies"}]},"item_type_id":"2","owner":"2","path":["36"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-06-29"},"publish_date":"2020-06-29","publish_status":"0","recid":"884","relation_version_is_last":true,"title":["A Simple and High-Throughput ELISA-Based Neutralization Assay for the Determination of Anti-Flavivirus Neutralizing Antibodies"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T04:11:44.007164+00:00"}