@article{oai:nagasaki-u.repo.nii.ac.jp:00000886,
 author = {Kodama, Yukinobu and Nakashima, Mikiro and Nagahara, Tadayuki and Oyama, Natsuko and Hashizume, Junya and Nakagawa, Hiroo and Harasawa, Hitomi and Muro, Takahiro and Kurosaki, Tomoaki and Yamashita, Chikamasa and Hashida, Mitsuru and Kitahara, Takashi and Sasaki, Hitoshi and Kawakami, Shigeru and Nakamura, Tadahiro},
 issue = {6},
 journal = {Pharmaceutics},
 month = {Jun},
 note = {The present study investigated a pulmonary delivery system of plasmid DNA (pDNA) and its application to melanoma DNA vaccines. pCMV-Luc, pEGFP-C1, and pZsGreen were used as a model pDNA to evaluate transfection efficacy after inhalation in mice. Naked pDNA and a ternary complex, consisting of pDNA, dendrigraft poly-L-lysine (DGL), and γ-polyglutamic acid (γ- PGA), both showed strong gene expression in the lungs after inhalation. The transgene expression was detected in alveolar macrophage-rich sites by observation using multi-color deep imaging. On the basis of these results, we used pUb-M, which expresses melanoma-related antigens (ubiquitinated murine melanoma gp100 and tyrosinase-related protein 2 (TRP2) peptide epitopes), as DNA vaccine for melanoma. The inhalation of naked pUb-M and its ternary complex significantly inhibited the metastasis of B16-F10 cells, a melanoma cell line, in mice. The levels of the inflammatory cytokines, such as TNF-α, IFN-γ, and IL-6, which enhance Th1 responses, were higher with the pUb-M ternary complex than with naked pUb-M and pEGFP-C1 ternary complex as control. In conclusion, we clarified that the inhalation of naked pDNA as well as its ternary complex are a useful technique for cancer vaccination., Pharmaceutics, 12(6), art.no.540; 2020},
 title = {Development of a DNA Vaccine for Melanoma Metastasis by Inhalation Based on an Analysis of Transgene Expression Characteristics of Naked pDNA and a Ternary Complex in Mouse Lung Tissues},
 volume = {12},
 year = {2020}
}