{"created":"2023-05-15T16:29:56.511357+00:00","id":886,"links":{},"metadata":{"_buckets":{"deposit":"09c3999c-4408-400f-afec-1824e265444a"},"_deposit":{"created_by":2,"id":"886","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"886"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00000886","sets":["8:9"]},"author_link":["4230","4238","4240","4243","4241","4239","4231","4234","4237","4232","4235","4236","4244","4242","4233"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-06-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageStart":"540","bibliographicVolumeNumber":"12","bibliographic_titles":[{"bibliographic_title":"Pharmaceutics"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The present study investigated a pulmonary delivery system of plasmid DNA (pDNA) and its application to melanoma DNA vaccines. pCMV-Luc, pEGFP-C1, and pZsGreen were used as a model pDNA to evaluate transfection efficacy after inhalation in mice. Naked pDNA and a ternary complex, consisting of pDNA, dendrigraft poly-L-lysine (DGL), and γ-polyglutamic acid (γ- PGA), both showed strong gene expression in the lungs after inhalation. The transgene expression was detected in alveolar macrophage-rich sites by observation using multi-color deep imaging. On the basis of these results, we used pUb-M, which expresses melanoma-related antigens (ubiquitinated murine melanoma gp100 and tyrosinase-related protein 2 (TRP2) peptide epitopes), as DNA vaccine for melanoma. The inhalation of naked pUb-M and its ternary complex significantly inhibited the metastasis of B16-F10 cells, a melanoma cell line, in mice. The levels of the inflammatory cytokines, such as TNF-α, IFN-γ, and IL-6, which enhance Th1 responses, were higher with the pUb-M ternary complex than with naked pUb-M and pEGFP-C1 ternary complex as control. In conclusion, we clarified that the inhalation of naked pDNA as well as its ternary complex are a useful technique for cancer vaccination.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Pharmaceutics, 12(6), art.no.540; 2020","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/pharmaceutics12060540","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution(CC BY) license (http://creativecommons.org/licenses/by/4.0/)."}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"19994923","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kodama, Yukinobu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakashima, Mikiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nagahara, Tadayuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Oyama, Natsuko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hashizume, Junya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakagawa, Hiroo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Harasawa, Hitomi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Muro, Takahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kurosaki, Tomoaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamashita, Chikamasa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hashida, Mitsuru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kitahara, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sasaki, Hitoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawakami, Shigeru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamura, Tadahiro"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-18"}],"displaytype":"detail","filename":"Pharmceutics12_540.pdf","filesize":[{"value":"7.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Pharmceutics12_540.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/886/files/Pharmceutics12_540.pdf"},"version_id":"f6f57327-43f7-4aa4-88e5-bf70461bd6c4"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"DNA vaccine","subitem_subject_scheme":"Other"},{"subitem_subject":"Gene delivery","subitem_subject_scheme":"Other"},{"subitem_subject":"Inhalation","subitem_subject_scheme":"Other"},{"subitem_subject":"Melanoma","subitem_subject_scheme":"Other"},{"subitem_subject":"Ternary complex","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Development of a DNA Vaccine for Melanoma Metastasis by Inhalation Based on an Analysis of Transgene Expression Characteristics of Naked pDNA and a Ternary Complex in Mouse Lung Tissues","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Development of a DNA Vaccine for Melanoma Metastasis by Inhalation Based on an Analysis of Transgene Expression Characteristics of Naked pDNA and a Ternary Complex in Mouse Lung Tissues"}]},"item_type_id":"2","owner":"2","path":["9"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-06-29"},"publish_date":"2020-06-29","publish_status":"0","recid":"886","relation_version_is_last":true,"title":["Development of a DNA Vaccine for Melanoma Metastasis by Inhalation Based on an Analysis of Transgene Expression Characteristics of Naked pDNA and a Ternary Complex in Mouse Lung Tissues"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T04:11:43.185100+00:00"}