@article{oai:nagasaki-u.repo.nii.ac.jp:00009243,
 author = {原, 研治 and 槌本, 六秀 and 橘, 勝康 and 長富, 潔 and 古場, 久代 and 石原, 忠},
 journal = {長崎大学水産学部研究報告, Bulletin of the Faculty of Fisheries, Nagasaki University},
 month = {Dec},
 note = {We investigated the preparation of macrophage monolayer from carp peritoneal exudate cells (PEC) to clarify the biosynthesis and processing of cathepsins. PEC collected at 24h interval after elicitation with 6% sodium caseinate or thioglycolate medium were transferred to culture dish in RPMI 1640 (RPMI), and the cells were incubated in CO₂ incubator for 2h. To distinguish macrophage and neutrophil, the adherent cells washed with RPMI to remove non-adherent cells were analyzed histochemically by the staining of two different esterase activities. The number of PEC was maximally increased at 3 days after injection with 15 ml of sodium caseinate, whereas that of macrophages was at 4 days after injection. Most. (about 90%) of cells cultured for 24h were positive for α-Naphthyl butyrate esterase activity but they were not positive for Naphthol AS-D chloroacetate esterase activity, confirming them to be macrophage. They were able to culture for 48h in RPMI containing 5% heat inactivated calf serum. It was possible to prepare two culture dishes of macrophage monolayer (1.33 x 10⁵/cm²) from one carp (800-900g) by this method., 崎大学水産学部研究報告, v.74･75, pp.51-57; 1993},
 pages = {51--57},
 title = {コイ腹腔滲出細胞からのマクロファージの調製},
 volume = {74･75},
 year = {1993}
}