{"created":"2023-05-15T16:36:12.006272+00:00","id":9243,"links":{},"metadata":{"_buckets":{"deposit":"595e8665-3924-4f7f-af28-56fdc7f9b4e6"},"_deposit":{"created_by":2,"id":"9243","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"9243"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00009243","sets":["50:78:79:682"]},"author_link":["37006","37004","37010","37002","37000","37003","36999","37001","37007","37008","37009","37005"],"item_3_alternative_title_19":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Preparation of Macrophage Monolayer from Carp Peritoneal Exudate Cells"}]},"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1993-12","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"57","bibliographicPageStart":"51","bibliographicVolumeNumber":"74･75","bibliographic_titles":[{"bibliographic_title":"長崎大学水産学部研究報告"},{"bibliographic_title":"Bulletin of the Faculty of Fisheries, Nagasaki University","bibliographic_titleLang":"en"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We investigated the preparation of macrophage monolayer from carp peritoneal exudate cells (PEC) to clarify the biosynthesis and processing of cathepsins. PEC collected at 24h interval after elicitation with 6% sodium caseinate or thioglycolate medium were transferred to culture dish in RPMI 1640 (RPMI), and the cells were incubated in CO₂ incubator for 2h. To distinguish macrophage and neutrophil, the adherent cells washed with RPMI to remove non-adherent cells were analyzed histochemically by the staining of two different esterase activities. The number of PEC was maximally increased at 3 days after injection with 15 ml of sodium caseinate, whereas that of macrophages was at 4 days after injection. Most. (about 90%) of cells cultured for 24h were positive for α-Naphthyl butyrate esterase activity but they were not positive for Naphthol AS-D chloroacetate esterase activity, confirming them to be macrophage. They were able to culture for 48h in RPMI containing 5% heat inactivated calf serum. It was possible to prepare two culture dishes of macrophage monolayer (1.33 x 10⁵/cm²) from one carp (800-900g) by this method.","subitem_description_type":"Abstract"}]},"item_3_description_64":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"崎大学水産学部研究報告, v.74･75, pp.51-57; 1993","subitem_description_type":"Other"}]},"item_3_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"37005","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Hara, Kenji"}]},{"nameIdentifiers":[{"nameIdentifier":"37006","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Tsuchimoto, Mutsuhide"}]},{"nameIdentifiers":[{"nameIdentifier":"37007","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Tachibana, Katsuyasu"}]},{"nameIdentifiers":[{"nameIdentifier":"37008","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Osatomi, Kiyoshi"}]},{"nameIdentifiers":[{"nameIdentifier":"37009","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Koba, Hisayo"}]},{"nameIdentifiers":[{"nameIdentifier":"37010","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Ishihara, Tadashi"}]}]},"item_3_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"長崎大学水産学部"}]},"item_3_relation_29":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"40002770932","subitem_relation_type_select":"NAID"}}]},"item_3_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00178473","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"05471427","subitem_source_identifier_type":"ISSN"}]},"item_3_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"原, 研治"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"槌本, 六秀"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"橘, 勝康"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"長富, 潔"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"古場, 久代"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"石原, 忠"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-22"}],"displaytype":"detail","filename":"suisan74-75_051.pdf","filesize":[{"value":"5.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"suisan74-75_051.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/9243/files/suisan74-75_051.pdf"},"version_id":"a42b61f0-cfb6-4e5f-867d-547ac3996133"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"マクロファージ","subitem_subject_scheme":"Other"},{"subitem_subject":"macrophage","subitem_subject_scheme":"Other"},{"subitem_subject":"腹腔滲出細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"peritoneal exudate cells","subitem_subject_scheme":"Other"},{"subitem_subject":"好中球","subitem_subject_scheme":"Other"},{"subitem_subject":"neutrophil","subitem_subject_scheme":"Other"},{"subitem_subject":"コイ","subitem_subject_scheme":"Other"},{"subitem_subject":"carp","subitem_subject_scheme":"Other"},{"subitem_subject":"非特異性エステラーゼ","subitem_subject_scheme":"Other"},{"subitem_subject":"non-specific esterase","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"コイ腹腔滲出細胞からのマクロファージの調製","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"コイ腹腔滲出細胞からのマクロファージの調製"}]},"item_type_id":"3","owner":"2","path":["682"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-11-26"},"publish_date":"2012-11-26","publish_status":"0","recid":"9243","relation_version_is_last":true,"title":["コイ腹腔滲出細胞からのマクロファージの調製"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T01:50:29.561956+00:00"}