@article{oai:nagasaki-u.repo.nii.ac.jp:00009304,
 author = {Chihara, Kohki and Kishikawa, Naoya and Ohyama, Kaname and Nakashima, Kenichiro and Kuroda, Naotaka},
 issue = {9},
 journal = {Analytical and Bioanalytical Chemistry},
 month = {Jul},
 note = {The Petasis reaction is the multi-component reaction of a carbonyl compound, amine, and arylboronic acid to form an α-amino acid or a β-aminoalcohol. In this work, as the first analytical application of the Petasis reaction, a highperformance liquid chromatographic (HPLC) method with fluorescence detection was developed for determination of glyoxylic acid. The glyoxylic acid was derivatized with 1- pyreneboronic acid, as fluorescent arylboronic acid, in the presence of N-methylbutylamine, as amine, to give a fluorescent α-amino acid. HPLC separation of the fluorescent derivative was performed within 30 min on an octyl column eluted with a gradient prepared from acetonitrile and 50 mmol L -1 acetate buffer (pH 4.0). The detection limit (S/N03) for glyoxylic acid was 5.0 nmol L -1 (20 fmol/injection). The method can be used to determine the concentration of glyoxylic acid in human urine without interference from biological components., Analytical and Bioanalytical Chemistry, 403(9), pp.2765-2770; 2012},
 pages = {2765--2770},
 title = {Determination of glyoxylic acid in urine by liquid chromatography with fluorescence detection, using a novel derivatization procedure based on the Petasis reaction},
 volume = {403},
 year = {2012}
}