{"created":"2023-05-15T16:36:17.304263+00:00","id":9304,"links":{},"metadata":{"_buckets":{"deposit":"339bfe8a-a9c5-4d9e-aeb2-67ad5fd7ce18"},"_deposit":{"created_by":2,"id":"9304","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"9304"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00009304","sets":["73:74"]},"author_link":["37396","37395","37394","37398","37397"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"9","bibliographicPageEnd":"2770","bibliographicPageStart":"2765","bibliographicVolumeNumber":"403","bibliographic_titles":[{"bibliographic_title":"Analytical and Bioanalytical Chemistry"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The Petasis reaction is the multi-component reaction of a carbonyl compound, amine, and arylboronic acid to form an α-amino acid or a β-aminoalcohol. In this work, as the first analytical application of the Petasis reaction, a highperformance liquid chromatographic (HPLC) method with fluorescence detection was developed for determination of glyoxylic acid. The glyoxylic acid was derivatized with 1- pyreneboronic acid, as fluorescent arylboronic acid, in the presence of N-methylbutylamine, as amine, to give a fluorescent α-amino acid. HPLC separation of the fluorescent derivative was performed within 30 min on an octyl column eluted with a gradient prepared from acetonitrile and 50 mmol L -1 acetate buffer (pH 4.0). The detection limit (S/N03) for glyoxylic acid was 5.0 nmol L -1 (20 fmol/injection). The method can be used to determine the concentration of glyoxylic acid in human urine without interference from biological components.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Analytical and Bioanalytical Chemistry, 403(9), pp.2765-2770; 2012","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Verlag"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1007/s00216-012-6036-1","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© Springer-Verlag 2012."},{"subitem_rights":"The final publication is available at www.springerlink.com."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"16182642","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"16182650","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Chihara, Kohki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kishikawa, Naoya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohyama, Kaname"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakashima, Kenichiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kuroda, Naotaka"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-22"}],"displaytype":"detail","filename":"ABC403_2765.pdf","filesize":[{"value":"226.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ABC403_2765.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/9304/files/ABC403_2765.pdf"},"version_id":"172c24e3-3b56-46a2-b2e1-c7ff71df90c4"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Acetate buffers","subitem_subject_scheme":"Other"},{"subitem_subject":"Amino alcohols","subitem_subject_scheme":"Other"},{"subitem_subject":"Analytical applications","subitem_subject_scheme":"Other"},{"subitem_subject":"Arylboronic acids","subitem_subject_scheme":"Other"},{"subitem_subject":"Biological components","subitem_subject_scheme":"Other"},{"subitem_subject":"Derivatization procedures","subitem_subject_scheme":"Other"},{"subitem_subject":"Detection limits","subitem_subject_scheme":"Other"},{"subitem_subject":"Fluorescence derivatization","subitem_subject_scheme":"Other"},{"subitem_subject":"Fluorescence detection","subitem_subject_scheme":"Other"},{"subitem_subject":"Glyoxylic acids","subitem_subject_scheme":"Other"},{"subitem_subject":"HPLC separation","subitem_subject_scheme":"Other"},{"subitem_subject":"Human urine","subitem_subject_scheme":"Other"},{"subitem_subject":"Multi-component reactions","subitem_subject_scheme":"Other"},{"subitem_subject":"Petasis reaction","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Determination of glyoxylic acid in urine by liquid chromatography with fluorescence detection, using a novel derivatization procedure based on the Petasis reaction","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Determination of glyoxylic acid in urine by liquid chromatography with fluorescence detection, using a novel derivatization procedure based on the Petasis reaction"}]},"item_type_id":"2","owner":"2","path":["74"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-11-30"},"publish_date":"2012-11-30","publish_status":"0","recid":"9304","relation_version_is_last":true,"title":["Determination of glyoxylic acid in urine by liquid chromatography with fluorescence detection, using a novel derivatization procedure based on the Petasis reaction"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T01:49:14.746648+00:00"}