{"created":"2023-05-15T16:36:26.183890+00:00","id":9473,"links":{},"metadata":{"_buckets":{"deposit":"59938aa5-6e35-4bb7-a555-0d54ae05d890"},"_deposit":{"created_by":2,"id":"9473","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"9473"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00009473","sets":["35:36"]},"author_link":["38238","38237","38236","38235","38234","38232","38233"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-07-27","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"7","bibliographicPageStart":"e41703","bibliographicVolumeNumber":"7","bibliographic_titles":[{"bibliographic_title":"PLoS ONE"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Introduction: Class I HLA's polymorphism has hampered CTL epitope mapping with laborious experiments. Objectives are 1) to evaluate the novel in silico model in predicting previously reported epitopes in comparison with existing program, and 2) to apply the model to predict optimal epitopes with HLA using experimental results. Materials and Methods: We have developed a novel in silico epitope prediction method, based on HLA crystal structure and a peptide docking simulation model, calculating the peptide-HLA binding affinity at four amino acid residues in each terminal. It was applied to predict 52 HIV best-defined CTL epitopes from 15-mer overlapping peptides, and its predictive ability was compared with the HLA binding motif-based program of HLArestrictor. It was then used to predict HIV-1 Gag optimal epitopes from previous ELISpot results. Results: 43/52 (82.7%) epitopes were detected by the novel model, whereas 37 (71.2%) by HLArestrictor. We also found a significant reduction in epitope detection rates for longer epitopes in HLArestrictor (p = 0.027), but not in the novel model. Improved epitope prediction was also found by introducing both models, especially in specificity (p<0.001). Eight peptides were predicted as novel, immunodominant epitopes in both models. Discussion: This novel model can predict optimal CTL epitopes, which were not detected by an existing program. This model is potentially useful not only for narrowing down optimal epitopes, but predicting rare HLA alleles with less information. By introducing different principal models, epitope prediction will be more precise.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"PLoS ONE, 7(7), e41703; 2012","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Public Library of Science"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1371/journal.pone.0041703","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© Mori et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"19326203","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Mori, Masahiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsuki, Kei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Maekawa, Tomoyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tanaka, Mari"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sriwanthana, Busarawan"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yokoyama, Masaru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ariyoshi, Koya"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-22"}],"displaytype":"detail","filename":"PLoS7_41703.pdf","filesize":[{"value":"165.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PLoS7_41703.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/9473/files/PLoS7_41703.pdf"},"version_id":"afb127cf-0d9b-40a4-9e17-eb72f2ddf7d0"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Development of a Novel In Silico Docking Simulation Model for the Fine HIV-1 Cytotoxic T Lymphocyte Epitope Mapping","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Development of a Novel In Silico Docking Simulation Model for the Fine HIV-1 Cytotoxic T Lymphocyte Epitope Mapping"}]},"item_type_id":"2","owner":"2","path":["36"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-12-21"},"publish_date":"2012-12-21","publish_status":"0","recid":"9473","relation_version_is_last":true,"title":["Development of a Novel In Silico Docking Simulation Model for the Fine HIV-1 Cytotoxic T Lymphocyte Epitope Mapping"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T01:45:10.608748+00:00"}