{"created":"2023-05-15T16:36:26.226440+00:00","id":9474,"links":{},"metadata":{"_buckets":{"deposit":"d04e99ed-514f-4014-b8c2-97e4f8cb8ac1"},"_deposit":{"created_by":2,"id":"9474","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"9474"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00009474","sets":["35:36"]},"author_link":["38243","38242","38246","38241","38245","38244","38240","38239"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-07-27","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"7","bibliographicPageStart":"e41696","bibliographicVolumeNumber":"7","bibliographic_titles":[{"bibliographic_title":"PLoS ONE"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Introduction: The design of a globally effective vaccine rests on the identification of epitopes capable of eliciting effective cytotoxic T lymphocyte (CTL) responses across multiple HIV clades in different populations. This study aims to discern the effect of HLA polymorphisms and the cross-clade reactivity or clade-specificity of epitopes in Thailand where HIV-1 CRF01_AE is circulating. Materials and Methods: 14 peptides based on consensus HIV-1 CRF01_AE amino acid sequences were designed for use in IFN-γ ELISpot assays and 51Cr release assays among 66 HIV-1 CRF01_AE-infected Thai patients. For ELISpot responders carrying HLA alleles currently unknown to restrict CRF01_AE epitopes, in silico epitope-HLA prediction was performed. Results: 29/66 (43.9%) patients recognized at least one peptide. In total 79 responses were seen against all 14 peptides. 28/79 (35.4%) of the responses were in patients with HLA alleles previously reported to restrict CRF01_AE epitopes, 24/79 (30.4%) responses were in individuals with HLA alleles previously reported to restrict epitopes of HIV clades other than CRF01_AE, and the remaining 27/79 (34.2%) responses were not associated with HLA alleles previously known to restrict HIV epitopes. In silico epitope prediction detected 19 novel, epitope-HLA combinations, and 11/19 (57.9%) were associated with HLA-C alleles. We further confirmed a novel HLA restriction of a previously identified HIV-1 Gag epitope [p24 122-130: PPIPVGDIY (PY9)] by HLA-B*40:01 with a standard 51Cr release assay. Discussion: CTL recognition sites in HIV-1 Gag were similar among different clades but the HLA restriction differed in Thai patients. This disparity in HLA restriction along different populations illustrated the importance of clade- and population-specific HLA analysis prior to CTL vaccine design.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"PLoS ONE, 7(7), e41696; 2012","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Public Library of Science"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1371/journal.pone.0041696","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2012 Sriwanthana et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"19326203","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sriwanthana, Busarawan"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mori, Masahiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tanaka, Mari"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nishimura, Sei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miura, Toshiyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Pathipvanich, Panita"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sawanpanyalert, Pathom"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ariyoshi, Koya"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-22"}],"displaytype":"detail","filename":"PLoS7_41696.pdf","filesize":[{"value":"361.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PLoS7_41696.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/9474/files/PLoS7_41696.pdf"},"version_id":"8ef408ec-78df-4052-9edb-5d1b0382e9a9"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The Effect of HLA Polymorphisms on the Recognition of Gag Epitopes in HIV-1 CRF01_AE Infection","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The Effect of HLA Polymorphisms on the Recognition of Gag Epitopes in HIV-1 CRF01_AE Infection"}]},"item_type_id":"2","owner":"2","path":["36"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-12-21"},"publish_date":"2012-12-21","publish_status":"0","recid":"9474","relation_version_is_last":true,"title":["The Effect of HLA Polymorphisms on the Recognition of Gag Epitopes in HIV-1 CRF01_AE Infection"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T01:45:08.334051+00:00"}