{"created":"2023-05-15T16:29:59.201429+00:00","id":948,"links":{},"metadata":{"_buckets":{"deposit":"540b4c96-6293-455d-b709-211685e03b47"},"_deposit":{"created_by":2,"id":"948","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"948"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00000948","sets":["35:36"]},"author_link":["4485","4486","4484","4483"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-04-24","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"586","bibliographicPageStart":"573","bibliographicVolumeNumber":"101","bibliographic_titles":[{"bibliographic_title":"Journal of General Virology"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Bone marrow stromal cell antigen-2 (BST-2), also known as tetherin, is an interferon-inducible membrane-associated protein. It effectively targets enveloped viruses at the release step of progeny viruses from host cells, thereby restricting the further spread of viral infection. Junin virus (JUNV) is a member of Arenaviridae, which causes Argentine haemorrhagic fever that is associated with a high rate of mortality. In this study, we examined the effect of human BST-2 on the replication and propagation of JUNV. The production of JUNV Z-mediated virus-like particles (VLPs) was significantly inhibited by over-expression of BST-2. Electron microscopy analysis revealed that BST-2 functions by forming a physical link that directly retains VLPs on the cell surface. Infection using JUNV showed that infectious JUNV production was moderately inhibited by endogenous or exogenous BST-2. We also observed that JUNV infection triggers an intense interferon response, causing an upregulation of BST-2, in infected cells. However, the expression of cell surface BST-2 was reduced upon infection. Furthermore, the expression of JUNV nucleoprotein (NP) partially recovered VLP production from BST-2 restriction, suggesting that the NP functions as an antagonist against antiviral effect of BST-2. We further showed that JUNV NP also rescued the production of Ebola virus VP40-mediated VLP from BST-2 restriction as a broad spectrum BST-2 antagonist. To our knowledge, this is the first report showing that an arenavirus protein counteracts the antiviral function of BST-2.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Journal of general virology, 101(6), pp.573-586; 2020","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Microbiology soceity"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1099/jgv.0.001414","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2020 The Authors. Published by the Microbiology Society. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00221317","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"14652099","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Zadeh, Vahid Rajabali"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Urata, Shuzo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sakaguchi, Miako"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yasuda, Jiro"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-18"}],"displaytype":"detail","filename":"JGW101_573.pdf","filesize":[{"value":"4.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JGW101_573.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/948/files/JGW101_573.pdf"},"version_id":"a12fcea4-0edd-44ca-b2f5-9e34b49068a4"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Junin virus","subitem_subject_scheme":"Other"},{"subitem_subject":"Arenaviridae","subitem_subject_scheme":"Other"},{"subitem_subject":"human BST-2","subitem_subject_scheme":"Other"},{"subitem_subject":"innate immunity","subitem_subject_scheme":"Other"},{"subitem_subject":"Tetherin","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Human BST-2/tetherin inhibits Junin virus release from host cells and its inhibition is partially counteracted by viral nucleoprotein","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Human BST-2/tetherin inhibits Junin virus release from host cells and its inhibition is partially counteracted by viral nucleoprotein"}]},"item_type_id":"2","owner":"2","path":["36"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-07-17"},"publish_date":"2020-07-17","publish_status":"0","recid":"948","relation_version_is_last":true,"title":["Human BST-2/tetherin inhibits Junin virus release from host cells and its inhibition is partially counteracted by viral nucleoprotein"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T04:10:28.983575+00:00"}