{"created":"2023-05-15T16:36:27.860572+00:00","id":9511,"links":{},"metadata":{"_buckets":{"deposit":"6335f96b-d567-4ef3-830c-c2704099fe42"},"_deposit":{"created_by":2,"id":"9511","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"9511"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00009511","sets":["10:11"]},"author_link":["38411","38407","38410","38408","38412","38406","38413","38409","38405","38414"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-08-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"29","bibliographicPageEnd":"3882","bibliographicPageStart":"3875","bibliographicVolumeNumber":"18","bibliographic_titles":[{"bibliographic_title":"World Journal of Gastroenterology"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"AIM: To establish an animal model with human hepatocyte- repopulated liver for the study of liver cancer metastasis. METHODS: Cell transplantation into mouse livers was conducted using alpha-fetoprotein (AFP)-producing human gastric cancer cells (h-GCCs) and h-hepatocytes as donor cells in a transgenic mouse line expressing urokinase-type plasminogen activator (uPA) driven by the albumin enhancer/promoter crossed with a severe combined immunodeficient (SCID) mouse line (uPA/ SCID mice). Host mice were divided into two groups (A and B). Group A mice were transplanted with h-GCCs alone, and group B mice were transplanted with h-GCCs and h-hepatocytes together. The replacement index (RI), which is the ratio of transplanted h-GCCs and h-hepatocytes that occupy the examined area of a histological section, was estimated by measuring h-AFP and h-albumin concentrations in sera, respectively, as well as by immunohistochemical analyses of h-AFP and human cytokeratin 18 in histological sections. RESULTS: The h-GCCs successfully engrafted, repopulated, and colonized the livers of mice in group A (RI = 22.0% ± 2.6%). These mice had moderately differentiated adenocarcinomatous lesions with disrupted glandular structures, which is a characteristics feature of gastric cancers. The serum h-AFP level reached 211.0 ± 142.2 g/mL (range, 7.1-324.2 g/mL). In group B mice, the h-GCCs and h-hepatocytes independently engrafted, repopulated the host liver, and developed colonies (RI = 12.0% ± 6.8% and 66.0% ± 12.3%, respectively). h-GCC colonies also showed typical adenocarcinomatous glandular structures around the h-hepatocyte- colonies. These mice survived for the full 56 day-study and did not exhibit any metastasis of h-GCCs in the extrahepatic regions during the observational period. The mice with an h-hepatocyte-repopulated liver possessed metastasized h-GCCs and therefore could be a useful humanized liver animal model for studying liver cancer metastasis in vivo. CONCLUSION: A novel animal model of human liver cancer metastasis was established using the uPA/SCID mouse line. This model could be useful for in vivo testing of anti-cancer drugs and for studying the mechanisms of human liver cancer metastasis.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"World Journal of Gastroenterology, 18(29), pp.3875-3882; 2012","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"WJG Press"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3748/wjg.v18.i29.3875","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 1995-2012 Baishideng Publishing Group Co., Limited. Articles published by this Open-Access journal are distributed under the terms of the Creative Commons Attribution Non-commercial License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited, the use is non commercial and is otherwise in compliance with the license."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"10079327","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Fujiwara, Shinsuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fujioka, Hikaru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tateno, Chise"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Taniguchi, Ken"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ito, Masahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohishi, Hiroshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Utoh, Rie"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ishibashi, Hiromi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kanematsu, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshizato, Katsutoshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-22"}],"displaytype":"detail","filename":"WJG18_3875.pdf","filesize":[{"value":"899.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"WJG18_3875.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/9511/files/WJG18_3875.pdf"},"version_id":"201263e0-f2b6-4aba-9e56-7e25b617832d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Alpha-fetoprotein-producing gastric cancer cells","subitem_subject_scheme":"Other"},{"subitem_subject":"Liver cancer metastasis","subitem_subject_scheme":"Other"},{"subitem_subject":"Mouse with humanized liver","subitem_subject_scheme":"Other"},{"subitem_subject":"Urokinase-type plasminogen activator/severe combined immunodeficient mouse","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A novel animal model for in vivo study of liver cancer metastasis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A novel animal model for in vivo study of liver cancer metastasis"}]},"item_type_id":"2","owner":"2","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-12-27"},"publish_date":"2012-12-27","publish_status":"0","recid":"9511","relation_version_is_last":true,"title":["A novel animal model for in vivo study of liver cancer metastasis"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T01:44:12.186455+00:00"}