{"created":"2023-05-15T16:36:29.819004+00:00","id":9556,"links":{},"metadata":{"_buckets":{"deposit":"8d40a519-ab83-46ed-ac4e-eba6d57085ab"},"_deposit":{"created_by":2,"id":"9556","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"9556"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00009556","sets":["8:9"]},"author_link":["38556","38554","38565","38558","38553","38557","38562","38551","38560","38559","38552","38563","38564","38555","38561"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"202","bibliographicPageStart":"195","bibliographicVolumeNumber":"47","bibliographic_titles":[{"bibliographic_title":"Journal of Gastroenterology"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background Geranylgeranylacetone (GGA), an isoprenoid compound which includes retinoids, has been used orally as an anti-ulcer drug in Japan. GGA acts as a potent inducer of anti-viral gene expression by stimulating ISGF3 formation in human hepatoma cells. This drug has few side effects and reinforces the effect of IFN when administered in combination with peg-IFN and ribavirin. This study verified the anti-HCV activity of GGA in a replicon system. In addition, mechanisms of anti-HCV activity were examined in the replicon cells. Methods OR6 cells stably harboring the full-length genotype 1 replicon containing the Renilla luciferase gene, ORN/C-5B/KE, were used to examine the influence of the anti-HCV effect of GGA. After treatment, the cells were harvested with Renilla lysis reagent and then subjected to a luciferase assay according to the manufacturer's protocol. Result The results showed that GGA had anti-HCV activity. GGA induced anti-HCV replicon activity in a time- and dose-dependent manner. GGA did not activate the tyrosine 701 and serine 727 on STAT-1, and did not induce HSP-70 in OR6 cells. The anti-HCV effect depended on the GGA induced mTOR activity, not STAT-1 activity and PKR. An additive effect was observed with a combination of IFN and GGA. Conclusions GGA has mTOR dependent anti-HCV activity. There is a possibility that the GGA anti-HCV activity can be complimented by IFN. It will be necessary to examine the clinical effectiveness of the combination of GGA and IFN for HCV patients in the future.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Journal of Gastroenterology, 47(2), pp.195-202; 2012","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本消化器病学会"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1007/s00535-011-0481-z","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© The Japanese Society of Gastroenterology. All rights reserved."},{"subitem_rights":"The final publication is available at www.springerlink.com."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09441174","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"14355922","subitem_source_identifier_type":"ISSN"}]},"item_2_text_62":{"attribute_name":"出版者別言語","attribute_value_mlt":[{"subitem_text_value":"The Japanese Society of Gastroenterology"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takeshita, Shigeyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ichikawa, Tatsuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Taura, Naota"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miyaaki, Hisamitsu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsuzaki, Toshihisa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Otani, Masashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Muraoka, Toru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Akiyama, Motohisa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miuma, Satoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ozawa, Eisuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ikeda, Masanori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kato, Nobuyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Isomoto, Hajime"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takeshima, Fuminao"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakao, Kazuhiko"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-22"}],"displaytype":"detail","filename":"JouGas47_195.pdf","filesize":[{"value":"428.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JouGas47_195.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/9556/files/JouGas47_195.pdf"},"version_id":"f0af98e7-6c9e-42ac-8eaa-d0069ba404cd"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"GGA","subitem_subject_scheme":"Other"},{"subitem_subject":"HCV","subitem_subject_scheme":"Other"},{"subitem_subject":"Interferon","subitem_subject_scheme":"Other"},{"subitem_subject":"MTOR","subitem_subject_scheme":"Other"},{"subitem_subject":"STAT-1","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Geranylgeranylacetone has anti-hepatitis C virus activity via activation of mTOR in human hepatoma cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Geranylgeranylacetone has anti-hepatitis C virus activity via activation of mTOR in human hepatoma cells"}]},"item_type_id":"2","owner":"2","path":["9"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-01-09"},"publish_date":"2013-01-09","publish_status":"0","recid":"9556","relation_version_is_last":true,"title":["Geranylgeranylacetone has anti-hepatitis C virus activity via activation of mTOR in human hepatoma cells"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T01:43:00.367989+00:00"}