{"created":"2023-05-15T16:36:39.907320+00:00","id":9789,"links":{},"metadata":{"_buckets":{"deposit":"3e1bd26c-1328-4806-9b0c-7b0d6307ea99"},"_deposit":{"created_by":2,"id":"9789","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"9789"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00009789","sets":["27:28"]},"author_link":["39549","39548","39546","39544","39545","39547"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"427","bibliographicPageStart":"421","bibliographicVolumeNumber":"49","bibliographic_titles":[{"bibliographic_title":"Indian Journal of Biochemistry and Biophysics"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Proenzymes with various lengths of propeptides have been observed in GluV8 from Staphylococcus aureus and GluSE from S. epidermidis. However, the production mechanism of these proenzymes and roles of truncated propeptides have yet to be elucidated. Here we demonstrate that shortening of propeptide commonly occurs in an auto-catalytic manner in GluV8-family members, including those from coagulase negative Staphylococci and Enterococcus faecalis. Accompanied with propeptide shortening, the pro-mature junction (Asn/Ser-1-Val1) becomes more susceptible towards the hetero-catalytic maturation enzymes. The auto-catalytic propeptide truncation is not observed in Ser169Ala inert molecules of GluV8-family members. A faint proteolytic activity of proenzymes from Staphylococcus caprae and E. faecalis is detected. In addition, proteolytic activity of proenzyme of GluV8 carrying Arg-3AlaAsn-1 is demonstrated with synthetic peptide substrates LLE/Q-MCA. These results suggest that GluV8-family proenzymes with shortened propeptides intrinsically possess proteolytic activity and are involved in the propeptide shortening that facilitates the final hetero-catalytic maturation.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Indian Journal of Biochemistry and Biophysics, 49(6) pp.421-427; 2012","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"NISCAIR-CSIR, India"}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved."},{"subitem_rights":"This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 2.5 India."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03011208","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"09750959","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Rouf, S M A"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohara-Nemoto, Yuko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shimoyama, Y"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kimura, S"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ono, Toshio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nemoto, Takayuki K."}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-22"}],"displaytype":"detail","filename":"IJBB49_421.pdf","filesize":[{"value":"369.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"IJBB49_421.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/9789/files/IJBB49_421.pdf"},"version_id":"d128691e-1bcb-4f64-8c3c-bff735a5cb20"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Enterococcus faecalis","subitem_subject_scheme":"Other"},{"subitem_subject":"GluV8","subitem_subject_scheme":"Other"},{"subitem_subject":"Propeptide","subitem_subject_scheme":"Other"},{"subitem_subject":"Staphylococcus aureus","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Propeptide processing and proteolytic activity of proenzymes of the Staphylococcal and Enterococcal GluV8-family protease","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Propeptide processing and proteolytic activity of proenzymes of the Staphylococcal and Enterococcal GluV8-family protease"}]},"item_type_id":"2","owner":"2","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-01-31"},"publish_date":"2013-01-31","publish_status":"0","recid":"9789","relation_version_is_last":true,"title":["Propeptide processing and proteolytic activity of proenzymes of the Staphylococcal and Enterococcal GluV8-family protease"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T01:37:46.824300+00:00"}