@article{oai:nagasaki-u.repo.nii.ac.jp:00009928,
 author = {伊藤, 栄樹 and 飯塚, 昭二},
 journal = {長崎大学水産学部研究報告, Bulletin of the Faculty of Fisheries, Nagasaki University},
 month = {Aug},
 note = {The fluorometric method of measuring chlorophyll-a involves some difficulties at practical measurement by field scienists unfamiliar with chemical analysis, although this method has some advantages. One of the problems is the difficulty in isolating pure chlorophyll-a and/or phaeophytina. This paper describes a simplified method of isolation of chlorophyll-a and phaeophytin-a for determination of two constants (fph and acid factor) without any special equipment or aid of skilled technique. Three features of this simplified method are mentioned in this paper. The first is to use fresh "wakame" (Undaria pinnatifida, brown alga) containing chlorophyll-a and no chlorophyll-b which is otherwise difficult to separate from chlorophyll-a; the second is to determine the amount of phaeophytin-a by light absorption method in order to simplify chromatography treatment; and the third is to isolate chlorophyll-a by paper chromatography., 長崎大学水産学部研究報告, v.47, pp.1-4; 1979},
 pages = {1--4},
 title = {蛍光法によるクロロフィルa測定に必要な定数算出のための色素の単離法},
 volume = {47},
 year = {1979}
}