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Visualization of PAX7 protein dynamics in muscle satellite cells in a YFP knock-in-mouse line
http://hdl.handle.net/10069/38568
http://hdl.handle.net/10069/3856834893215-0e62-4fc6-84f7-26997f181139
名前 / ファイル | ライセンス | アクション |
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SkeletMus8_26.pdf (5.9 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-09-13 | |||||
タイトル | ||||||
タイトル | Visualization of PAX7 protein dynamics in muscle satellite cells in a YFP knock-in-mouse line | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Knock-in mouse | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Muscle regeneration | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Myogenesis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Pax7 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | YFP | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kitajima, Yasuo
× Kitajima, Yasuo× Ono, Yusuke |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background: Satellite cells are residential muscle stem cells that express a paired box protein, PAX7. Results: Here, we report a knock-in mouse line expressing a PAX7-enhanced yellow fluorescent protein (YFP) fusion protein that enables visualization of PAX7 protein dynamics in living satellite cells through YFP fluorescence. The YFP fluorescence signals in Pax7-YFP knock-in mice clearly recapitulated the endogenous expression of PAX7 protein in satellite cells. YFP+ satellite cells were efficiently isolated from muscle tissues by fluorescence-activated cell sorting. Homozygous Pax7-YFP knock-in mice (Pax7YFP/YFP) were viable, grew and regenerated muscle normally, and Pax7YFP/YFP mouse-derived satellite cells proliferated, differentiated, and self-renewed as efficiently as those from wild-type (Pax7+/+) mice. Conclusions: Taken together, our Pax7-YFP mouse line is a useful tool to aid the development of stem-cell-based therapies for muscle diseases. | |||||
書誌情報 |
Skeletal Muscle 巻 8, 号 1, p. art.no.26, 発行日 2018-08-24 |
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出版者 | ||||||
出版者 | BioMed Central Ltd. | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 20445040 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1186/s13395-018-0174-x | |||||
権利 | ||||||
権利情報 | c 2018 The Author(s).This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Skeletal Muscle, 8(1), art.no.26; 2018 |