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フローインジェクション-ルミノール化学発光検出によるブドウ種子抽出物及びポリフェノール類の活性酸素種消去能評価
http://hdl.handle.net/10069/21886
http://hdl.handle.net/10069/2188636da180d-6870-44a9-bd57-2a69f09b148c
名前 / ファイル | ライセンス | アクション |
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BunsekiKagaku55_931.pdf (357.7 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2009-05-18 | |||||
タイトル | ||||||
タイトル | フローインジェクション-ルミノール化学発光検出によるブドウ種子抽出物及びポリフェノール類の活性酸素種消去能評価 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | flow injection analysis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | chemiluminescent assay | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | grape seed extract | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | polyphenolics | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | reactive oxygen species | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
和田, 光弘
× 和田, 光弘× 加藤, 正之× 城戸, 浩胤× 中嶋, 弥穂子× 黒田, 直敬× 中島, 憲一郎 |
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著者別名 | ||||||
姓名 | Wada, Mitsuhiro | |||||
著者別名 | ||||||
姓名 | Katoh, Masayuki | |||||
著者別名 | ||||||
姓名 | Kido, Hirotsugu | |||||
著者別名 | ||||||
姓名 | Nakashima, Mihoko N. | |||||
著者別名 | ||||||
姓名 | Kuroda, Naotaka | |||||
著者別名 | ||||||
姓名 | Nakashima, Kenichiro | |||||
その他のタイトル | ||||||
その他のタイトル | Flow Injection Analysis with Luminol Chemiluminescence Detection for Evaluation of Quenching Effects of Grape Seed Extracts and Polyphenolics against Reactive Oxygen Species | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | フローインジェクション分析(FIA)-ルミノール化学発光検出による簡便・迅速な活性酸素種(ROS)に対する消去能の評価法を開発し,これを市販のブドウ種子抽出物及びそれらに含まれることが知られているポリフェノール類に適用した.ヒドロキシラジカル(^・OH),一重項酸素(^1O_2),パーオキシナイトライトCONOO^-)及びスーパーオキシドアニオン(O_2^-)等のROSとルミノールとの反応によって生じる化学発光に対して,抗酸化物質含有試料の添加による減少率をROS消去能とした.本FIAシステムは,1検体を1分以内に測定可能であり,各ROSにおける,抗酸化物質含有試料を未添加時のブランク発光の精度も良好であった(相対標準偏差?4.5%, n=5).用量-反応曲線から算出したシアニジンのEC_<50>値(n=3)はそれぞれ50±4ng/assay (^・OH), 148±8(^1O_2), 36±4(ONOO^-)及び95±8(O_2^-)であった.カルコンを除くペラルゴニジン,デルフィニジン及びレスベラトロール等のポリフェノール類もROSに対する強い消去能を示した.また,ブドウ種子抽出物でも同様なROS消去能が観察された. | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A simple and rapid FIA method for measurement of quenching effects of grape seed extracts and polyphenolics against reactive oxygen species (ROS) was developed. This method is based on the determination of luminol chemiluminescence generated with ROS such as hydroxyl radical (^・OH), singlet oxygen (^1O_2), peroxynitrite (ONOO^-) and superoxide anion (O_2^-). More than 1 sample/min could be measured with satisfactory precision. The total reagent amount in this method could be reduced to one tenth of that for our previous batch method. The grape seed extracts A and B were successfully evaluated by the proposed method. The EC_<50> of cyanidin as a representative polyphenolic against ^・OH, ^1O_2, ONOO^・ and O_2^- were 50±4, 148±8, 36±4, and 95±8ng/assay (n=3), respectively. Except for chalcone, the other polyphenolics such as pelargonidin, delphinidin and trans-resveratrol also showed the high quenching effects against ROS. | |||||
書誌情報 |
分析化学 en : BUNSEKI KAGAKU 巻 55, 号 12, p. 931-936, 発行日 2006-12-05 |
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出版者 | ||||||
出版者 | 日本分析化学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 05251931 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00222633 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.2116/bunsekikagaku.55.931 | |||||
権利 | ||||||
権利情報 | The Japan Society for Analytical Chemistry (c) 2006 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 分析化学, 55(12), pp. 931-936; 2006 |