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Secure Splenic Delivery of Plasmid DNA and Its Application to DNA Vaccine
http://hdl.handle.net/10069/34047
http://hdl.handle.net/10069/34047a12cb887-fcd6-4ae6-90f2-765fbf278790
名前 / ファイル | ライセンス | アクション |
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BPBul36_1800.pdf (1.8 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-01-09 | |||||
タイトル | ||||||
タイトル | Secure Splenic Delivery of Plasmid DNA and Its Application to DNA Vaccine | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA vaccine | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Gene delivery | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Melanoma | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Spleen | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Ternary complex | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kurosaki, Tomoaki
× Kurosaki, Tomoaki× Kodama, Yukinobu× Muro, Takahiro× Higuchi, Norihide× Nakamura, Tadahiro× Kitahara, Takashi× Miyakoda, Mana× Yui, Katsuyuki× Sasaki, Hitoshi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | In this experiment, we developed a novel safe and effective gene delivery vector coated with γ-polyglutamic acid (γ-PGA-coated complexes). The γ-PGA-coated complex was composed of chiseled spherical nano-particles with anionic charges. The plasmid DNA/polyethyleneimine complex (non-coated complex) showed high transgene efficiency in the spleen and lung after intravenous administration in mice, with high liver toxicity and lethality. On the other hand, γ-PGA-coated complex selectively showed high transgene efficiency in the spleen without such toxicity. Furthermore, the γ-PGA-coated complex highly accumulated and showed high gene expression in the marginal zone of the spleen. Those results strongly indicated that γ-PGA-coated complex was suitable as a DNA vaccine vector. We therefore applied γ-PGA-coated complex to melanoma DNA vaccine, pUb-M. The γ-PGA-coated complex containing pUb-M significantly inhibited the growth and metastasis of a melanoma cell line, B16-F10 cells. In conclusion, we developed a splenic gene vector, γ-PGA-coated complex, as a novel technology for clinical vaccination. | |||||
書誌情報 |
Biological and Pharmaceutical Bulletin 巻 36, 号 11, p. 1800-1806, 発行日 2013-11-01 |
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出版者 | ||||||
出版者 | 日本薬学会 | |||||
出版者別言語 | ||||||
Pharmaceutical Society of Japan | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09186158 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13475215 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1248/bpb.b13-00489 | |||||
権利 | ||||||
権利情報 | © 2013 The Pharmaceutical Society of Japan. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Biological and Pharmaceutical Bulletin, 36(11), pp.1800-1806; 2013 |