WEKO3
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They are characterized by overproduction of extracellular matrix (ECM) such as collagens. MicroRNAs (miRNAs) are noncoding small RNAs and negatively regulate protein expression. Several miRNAs that have critical roles in tissue fibrosis and ECM metabolism have been reported. However, regulation and function of miRNAs in keloid remain to be explored. The purpose of this study was to identify miRNAs involved in keloid pathogenesis. We performed miRNA microarray analysis to compare miRNA expression profiles between keloid-derived fibroblasts (KFs) and normal fibroblasts (NFs). In all, 7 upregulated and 20 downregulated miRNAs were identified. Among these, we focused on miR-196a, which showed the highest fold change. Overexpression or knockdown of miR-196a led to a decreased or increased level of secreted type I/III collagens, respectively. Reporter analysis showed direct binding of miR-196a to the 3′ untranslated region (UTR) of COL1A1 and COL3A1. 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miR-196a Downregulation Increases the Expression of Type I and III Collagens in Keloid Fibroblasts
http://hdl.handle.net/10069/29204
http://hdl.handle.net/10069/292044cde25d7-c27f-4c09-9b0e-feddd1523d46
名前 / ファイル | ライセンス | アクション |
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JID132_1597.pdf (3.6 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2013-01-01 | |||||
タイトル | ||||||
タイトル | miR-196a Downregulation Increases the Expression of Type I and III Collagens in Keloid Fibroblasts | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | collagen type 1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | collagen type 3 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | corticosteroid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | microRNA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | microRNA 196a | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | tranilast | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | unclassified drug | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 3' untranslated region | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | acne | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | adolescent | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | adult | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | aged | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | article | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | blister | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | clinical article | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | controlled study | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | down regulation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | electron therapy | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | female | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | fibroblast | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | human | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | human cell | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | human tissue | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | keloid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | male | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | microarray analysis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | pathogenesis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | priority journal | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | protein expression | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | RNA binding | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | skin surgery | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | upregulation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Adolescent | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Aged, 80 and over | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Collagen Type I | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Collagen Type III | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | CpG Islands | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Dermis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA Methylation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Down-Regulation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Female | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Fibroblasts | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Luciferases | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Middle Aged | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Primary Cell Culture | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Promoter Regions | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Genetic | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Real-Time Polymerase Chain Reaction | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Young Adult | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kashiyama, Kazuya
× Kashiyama, Kazuya× Mitsutake, Norisato× Matsuse, Michiko× Ogi, Tomoo× Saenko, Vladimir A× Ujifuku, Kenta× Utani, Atsushi× Hirano, Akiyoshi× Yamashita, Shunichi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Keloids are a fibroproliferative disease due to abnormal wound healing process after skin injury. They are characterized by overproduction of extracellular matrix (ECM) such as collagens. MicroRNAs (miRNAs) are noncoding small RNAs and negatively regulate protein expression. Several miRNAs that have critical roles in tissue fibrosis and ECM metabolism have been reported. However, regulation and function of miRNAs in keloid remain to be explored. The purpose of this study was to identify miRNAs involved in keloid pathogenesis. We performed miRNA microarray analysis to compare miRNA expression profiles between keloid-derived fibroblasts (KFs) and normal fibroblasts (NFs). In all, 7 upregulated and 20 downregulated miRNAs were identified. Among these, we focused on miR-196a, which showed the highest fold change. Overexpression or knockdown of miR-196a led to a decreased or increased level of secreted type I/III collagens, respectively. Reporter analysis showed direct binding of miR-196a to the 3′ untranslated region (UTR) of COL1A1 and COL3A1. In conclusion, we demonstrate for the first time that miRNA expression profile is altered in KFs compared with NFs. Downregulation of miR-196a may be one of the mechanisms by which collagens are highly deposited in keloid tissues. Our findings suggest that miR-196a could be a new therapeutic target for keloid lesions. | |||||
書誌情報 |
Journal of Investigative Dermatology 巻 132, 号 6, p. 1597-1604, 発行日 2012-06 |
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出版者 | ||||||
出版者 | Nature Publishing Group | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0022202X | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 15231747 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1038/jid.2012.22 | |||||
権利 | ||||||
権利情報 | © 2012 The Society for Investigative Dermatology. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of Investigative Dermatology, 132(6), pp.1597-1604; 2012 |