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Purification, molecular cloning, and some properties of a manganese-containing superoxide dismutase from Japanese flounder (Paralichthys olivaceus).
http://hdl.handle.net/10069/24898
http://hdl.handle.net/10069/24898dea9c80c-1a85-428a-8bb8-e09a250861c8
名前 / ファイル | ライセンス | アクション |
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CBPb158_289.pdf (283.7 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2011-04-15 | |||||
タイトル | ||||||
タイトル | Purification, molecular cloning, and some properties of a manganese-containing superoxide dismutase from Japanese flounder (Paralichthys olivaceus). | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Amino acid sequence | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | CDNA sequence | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Hepatopancreas | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Japanese flounder | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Manganese-superoxide dismutase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Mitochondria-targeting signal | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Purification | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Wang, Yajun
× Wang, Yajun× Osatomi, Kiyoshi× Nagatomo, Yoshimi× Yoshida, Asami× Hara, Kenji |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Manganese-superoxide dismutase (Mn-SOD) from Japanese flounder (Paralichthys olivaceus) hepatopancreas has been purified with high purification (781-fold) and recovery (10.8%). The molecular mass of the purified enzyme was estimated to be 26kDa by SDS-PAGE under reducing conditions. In activity staining by native-PAGE, the Japanese flounder Mn-SOD gave three active bands and exhibited KCN-insensitive activity. In addition, the electrophoretic mobility of this enzyme was observed to be faster than that of Japanese flounder Cu,Zn-SOD. On the other hand, the N-terminal amino acid sequence of this Mn-SOD was determined to be 16 amino acid residues, and the sequence showed high homology to other Mn-SODs but not Japanese flounder Cu,Zn-SOD. Analysis of nucleotide and deduced amino acid sequences revealed that the Mn-SOD cDNA consisted of a 64bp 5'-non-coding region, a 675bp open reading frame encoding 225 amino acids, and a 465bp 3'-non-coding region. The first 27 amino acids containing a mitochondria-targeting signal were highly conserved among other Mn-SODs. | |||||
書誌情報 |
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 巻 158, 号 4, p. 289-296, 発行日 2011-04 |
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出版者 | ||||||
出版者 | Elsevier Inc. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 10964959 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 18791107 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11045017 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 21199678 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.cbpb.2010.12.007 | |||||
権利 | ||||||
権利情報 | Copyright © 2011 Elsevier Inc. All rights reserved. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 158(4), pp.289-296; 2011 |