WEKO3
アイテム
Enhanced response to mouse thyroid-stimulating hormone (TSH) receptor immunization in TSH receptor-knockout mice.
http://hdl.handle.net/10069/25635
http://hdl.handle.net/10069/25635b2a9b8d4-1622-4987-9ee7-c4ec7862cf71
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Endo151_4047.pdf (937.0 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2011-09-01 | |||||
| タイトル | ||||||
| タイトル | Enhanced response to mouse thyroid-stimulating hormone (TSH) receptor immunization in TSH receptor-knockout mice. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Graves’ disease | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | thyrotropin receptor | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | knockout mice | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | thyroid autoimmunity | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | cross-reactivity | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Nakahara, Mami
× Nakahara, Mami× Mitsutake, Norisato× Sakamoto, Hikaru× Chen, Chun-Rong× Rapoport, Basil× McLachlan, Sandra M× Nagayama, Yuji |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Graves-like hyperthyroidism is induced in BALB/c mice by immunization with adenovirus expressing the human TSH receptor (TSHR) A-subunit (amino acids 1-289). However, because of nonidentity between the human and mouse TSHR ( approximately 87% amino acid homology), we compared the responses of mice immunized with adenoviruses expressing either the mouse or the human TSHR A-subunit. Wild-type (wt) BALB/c mice immunized with the mouse A-subunit developed neither TSHR antibodies (measured by flow cytometry) nor thyroid lymphocytic infiltration. However, wt C57BL/6 mice developed sparse intrathyroidal lymphocyte infiltration without antibody production. Depletion of naturally occurring regulatory CD4(+)CD25(+) T cells had little effect. These results indicate the inability to break tolerance to the mouse TSHR in wt mice. In contrast, TSHR knockout (KO) BALB/c mice generated mouse TSHR antibodies in response to mouse A-subunit immunization and augmented human TSHR antibody response to human A-subunit immunization. Thyroid-stimulating antibody titers measured in a functional bioassay were comparable in human A-subunit immunized wt mice and in TSHR KO mice immunized with either the mouse or human A-subunit. In conclusion, immune response to the mouse TSHR is readily induced in TSHR KO but not in wt mice. Only in the former does immunization with adenovirus expressing the mouse A-subunit generate antibodies capable of activating the mouse TSHR. TSHR KO mice are, therefore, of value for future studies dissecting the autoimmune response to the mouse TSHR. | |||||
| 書誌情報 |
Endocrinology 巻 151, 号 8, p. 4047-4054, 発行日 2010-08 |
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| 出版者 | ||||||
| 出版者 | The Endocrine Society | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00137227 | |||||
| EISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 19457170 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00635074 | |||||
| PubMed番号 | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 20555026 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1210/en.2010-0315 | |||||
| 権利 | ||||||
| 権利情報 | Copyright © 2010 by The Endocrine Society | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| 関係URI | ||||||
| 関連名称 | http://hdl.handle.net/10069/26720 | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Endocrinology, 151(8), pp.4047-4054; 2010 | |||||
