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In Situ Hybridization Techniques for Electron Microscopy
http://hdl.handle.net/10069/16057
http://hdl.handle.net/10069/160572a54f238-2b47-4d8c-b513-8ee0f2221f5d
名前 / ファイル | ライセンス | アクション |
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acta41_03_01_t.pdf (1.5 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2008-03-13 | |||||
タイトル | ||||||
タイトル | In Situ Hybridization Techniques for Electron Microscopy | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | in situ hybridization | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | electron microscopy | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | backscattered electron imaging | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | RNA | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
Izumi, Shin-Ichi
× Izumi, Shin-Ichi× Nakane, Paulk. |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | In order for messenger RNA (mRNA) to be localized in cells and tissues by in situ hybridization at the electron microscopic level, the following conditions must be accomplished. The target mRNA must be retained in the specimen with well-preserved morphology. The labeled-nucleic acid probes must be accessible to the mRNA and make hybrids with the mRNA efficiently. The labels should be detectable by either transmission or scanning electron microscopes. To achieve these, appropriate techniques for the electron microscopic in situ hybridization (EMISH) for the target mRNA were developed and have been improved. This review will describe representative procedures of EMISH of mRNA and will make some comments on the procedures. | |||||
書誌情報 |
Acta medica Nagasakiensia 巻 41, 号 3-4, p. 1-7, 発行日 1996-12 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00016055 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00508430 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
sortkey | ||||||
P00001-P00007 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Acta medica Nagasakiensia. 1996, 41(3-4), p.1-7 |