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Optimization of pH Elution Conditions in Immune Complexome Analysis for Comprehensive Identification of Immune Complex Antigens
http://hdl.handle.net/10069/40407
http://hdl.handle.net/10069/40407f52f148e-e021-4eae-b3b1-77fb3b8f436c
名前 / ファイル | ライセンス | アクション |
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AnaSci36_1423.pdf (421.8 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-12-01 | |||||
タイトル | ||||||
タイトル | Optimization of pH Elution Conditions in Immune Complexome Analysis for Comprehensive Identification of Immune Complex Antigens | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Mass spectrometry | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | pH elution | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | papain-mediated elution | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | immune complex antigen | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | immunological diseases | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
AIBARA, Nozomi
× AIBARA, Nozomi× AIZAWA, Rika× NAKASHIMA, Mikiro× OHYAMA, Kaname |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The identification of antigens incorporated into immune complexes (IC-antigens) is important for studying the pathophysiology of immunological diseases. Immune complexome analysis identifies IC-antigens by analyzing ICs collected from biological fluids by IC-capturing beads. In this study, we optimized the method to improve its comprehensiveness while maintaining selectivity for IC-antigens by comparing the number of identified peptides (model IC experiment) or proteins (human pooled serum) eluted from Protein G beads using different pH solutions (pH 2.0 - 11.0). |
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書誌情報 |
Analytical Sciences 巻 36, 号 11, p. 1423-1426, 発行日 2020-11-10 |
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出版者 | ||||||
出版者 | 日本分析化学会 | |||||
出版者別言語 | ||||||
The Japan Society for Analytical Chemistry | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09106340 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13482246 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.2116/analsci.20P112 | |||||
権利 | ||||||
権利情報 | c 2020 by The Japan Society for Analytical Chemistry | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Analytical sciences 36(11), pp.1423-1426; 2020 |