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異なるDNA精製法が定量PCRによるEdwardsiella tardaの計数に与える影響
http://hdl.handle.net/10069/37991
http://hdl.handle.net/10069/379915971a810-4a6c-4187-8afd-5fb88763cfbd
名前 / ファイル | ライセンス | アクション |
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suisan99_2.pdf (1.7 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2018-04-06 | |||||
タイトル | ||||||
タイトル | 異なるDNA精製法が定量PCRによるEdwardsiella tardaの計数に与える影響 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 定量PCR | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 細菌計数 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA精製 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Edwardsiella tarda | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | quantitative PCR | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | bacterial count | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA purification | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
朝重, 紅音
× 朝重, 紅音× 菅, 向志郎× 金井, 欣也 |
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著者別名 | ||||||
姓名 | Tomoshige, Akane | |||||
著者別名 | ||||||
姓名 | Suga, Koushirou | |||||
著者別名 | ||||||
姓名 | Kanai, Kinya | |||||
その他のタイトル | ||||||
その他のタイトル | Effect of different DNA purification kits on counting the cell number of Edwardsiella tarda using quantitative PCR | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Quantitative PCR (qPCR) quantifies the template DNA from the number of threshold cycles (Ct value), but the probability of quantitative errors occurs due to the differences in recovery rates during DNA purification. In order to investigate the effect of different DNA purification kits in counting Edwardsiella tarda cells using qPCR, we compared two methods; the bacterial culture dilution method, in which the DNA is purified after 5 steps of a ten-fold serial dilution of E. tarda culture, and the DNA dilution method, which the DNA is diluted after purification with the optimum cell number of E. tarda. In both methods, we used three types of DNA purification kit: Chelex resin, Non-Silica membrane and Silica membrane. Our results showed the lowest Ct value and high DNA recovery rate were obtained using Chelex resin. Non-Silica membrane and Silica membrane Ct values in DNA dilution method were higher than that of Chelex resin. With the use of Non-Silica membrane, the calculated DNA using bacterial culture dilution method decreased by 67-87% compared to DNA dilution method at 1.0×10 4cells/μL of E. tarda. |
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書誌情報 |
長崎大学水産学部研究報告 en : Bulletin of the Faculty of Fisheries Nagasaki University 巻 99, p. 7-11, 発行日 2018-03 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 05471427 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00178473 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | 長崎大学水産学部 | |||||
出版者別言語 | ||||||
The Faculty of Fisheries, Nagasaki University | ||||||
sortkey | ||||||
02 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 長崎大学水産学部研究報告, 99, pp.7-11; 2018 |