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Development of multiplex serological assay for the detection of human African trypanosomiasis
http://hdl.handle.net/10069/36214
http://hdl.handle.net/10069/36214c64804c8-d54d-4a7b-bf1e-44c9afc75bae
名前 / ファイル | ライセンス | アクション |
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ISYO43_Nzou.pdf (852.3 kB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2016-03-16 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Development of multiplex serological assay for the detection of human African trypanosomiasis | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Human African trypanosomiasis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Sero-diagnosis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Multiplex assay | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Recombinant antigens | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Sero-surveillance | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
著者 |
Nzou, Samson Muuo
× Nzou, Samson Muuo |
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著者別名 | ||||||
姓名 | ンゾウ, サムソン ムウオ | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Human African trypanosomiasis (HAT) is a disease caused by Kinetoplastid infection. Serological tests are useful for epidemiological surveillance. The aim of this study was to develop a multiplex serological assay for HAT to assess the diagnostic value of selected HAT antigens for sero-epidemiological surveillance. We cloned loci encoding eight antigens from Trypanosoma brucei gambiense, expressed the genes in bacterial systems, and purified the resulting proteins. Antigens were subjected to Luminex multiplex assays using sera from HAT and VL patients to assess the antigens' immunodiagnostic potential. Among T. b. gambiense antigens, the 64-kDa and 65-kDa invariant surface glycoproteins (ISGs) and flagellar calcium binding protein (FCaBP) had high sensitivity for sera from T. b. gambiense patients, yielding AUC values of 0.871, 0.737 and 0.858 respectively in receiver operating characteristics (ROC) analysis. The ISG64, ISG65, and FCaBP antigens were partially cross-reactive to sera from Trypanosoma brucei rhodesiense patients. The GM6 antigen was cross-reactive to sera from T. b. rhodesiense patients as well as to sera from VL patients. Furthermore, heterogeneous antibody responses to each individual HAT antigen were observed. Testing for multiple HAT antigens in the same panel allowed specific and sensitive detection. Our results demonstrate the utility of applying multiplex assays for development and evaluation of HAT antigens for use in sero-epidemiological surveillance. |
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内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 長崎大学学位論文 学位記番号:博(医歯薬)乙第43号 学位授与年月日:平成28年2月3日 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Author: Samson Muuo Nzou, Yoshito Fujii, Masashi Miura, Matilu Mwau, Anne Wanjiru Mwangi, Makoto Itoh, Md. Abdus Salam, Shinjiro Hamano, Kenji Hirayama, Satoshi Kaneko | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Citation: Parasitology International, 65(2), pp.121-127; 2016 | |||||
書誌情報 |
Parasitology International 巻 65, 号 2, p. 121-127, 発行日 2016-02-03 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1383-5769 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.parint.2015.10.008 | |||||
権利 | ||||||
権利情報 | (C) 2015 The Authors. Published by Elsevier Ireland Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
その他のタイトル | ||||||
その他のタイトル | マルチプレックス法を用いたアフリカトリパノソーマ症の血清診断法の開発 | |||||
出版者 | ||||||
出版者 | Elsevier | |||||
関係URI | ||||||
識別子タイプ | HDL | |||||
関連識別子 | http://hdl.handle.net/10069/36277 | |||||
学位名 | ||||||
学位名 | 博士(医学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 17301 | |||||
学位授与機関名 | Nagasaki University (長崎大学) | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2016-02-03 | |||||
学位授与番号 | ||||||
学位授与番号 | 乙医歯薬第43号 | |||||
学位の種類 | ||||||
論文博士 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Nagasaki University (長崎大学), 博士(医学) (2016-02-03) |