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In Vitro Evaluation of Inhibitory Effect of Nuclear Factor-KappaB Activity by Small Interfering RNA on Pro-tumor Characteristics of M2-Like Macrophages
http://hdl.handle.net/10069/34195
http://hdl.handle.net/10069/341956cf5bf22-b155-49d2-9f1d-71963ea4574b
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
BPBul37_137.pdf (1.2 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2014-03-13 | |||||
| タイトル | ||||||
| タイトル | In Vitro Evaluation of Inhibitory Effect of Nuclear Factor-KappaB Activity by Small Interfering RNA on Pro-tumor Characteristics of M2-Like Macrophages | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Nuclear factor-kappaB | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Small interfering RNA | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Transfection | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Tumor-associated macrophage | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Kono, Yusuke
× Kono, Yusuke× Kawakami, Shigeru× Higuchi, Yuriko× Yamashita, Fumiyoshi× Hashida, Mitsuru |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Tumor-associated macrophages (TAMs) have an alternatively activated macrophage phenotype (M2) and promote cancer cell proliferation, angiogenesis and metastasis. Nuclear factor-kappaB (NF-κB) is one of the master regulators of macrophage polarization. Here, we investigated the effect of inhibition of NF-κB activity by small interfering RNA (siRNA) on the pro-tumor response of macrophages located in the tumor microenvironment in vitro. We used mouse peritoneal macrophages cultured in conditioned medium from colon-26 cancer cells as an in vitro TAM model (M2-like macrophages). Transfection of NF-κB (p50) siRNA into M2-like macrophages resulted in a significant decrease in the secretion of interleukin (IL)-10 (a T helper 2 (Th2) cytokine) and a significant increase of T helper 1 (Th1) cytokine production (IL-12, tumor necrosis factor-α, and IL-6). Furthermore, vascular endothelial growth factor production and matrix metalloproteinase-9 mRNA expression in M2-like macrophages were suppressed by inhibition of NF-κB expression with NF-κB (p50) siRNA. In addition, there was a reduction of arginase mRNA expression and increase in nitric oxide production. The cytokine secretion profiles of macrophages cultured in conditioned medium from either B16BL6 or PAN-02 cancer cells were also converted from M2 to classically activated (M1) macrophages by transfection of NF-κB (p50) siRNA. These results suggest that inhibition of NF-κB activity in M2-like macrophages alters their phenotype toward M1. | |||||
| 書誌情報 |
Biological and Pharmaceutical Bulletin 巻 37, 号 1, p. 137-144, 発行日 2014-01-01 |
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| 出版者 | ||||||
| 出版者 | 日本薬学会 | |||||
| 出版者別言語 | ||||||
| 値 | Pharmaceutical Society of Japan | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 09186158 | |||||
| EISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 13475215 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1248/bpb.b13-00659 | |||||
| 権利 | ||||||
| 権利情報 | c 2014 The Pharmaceutical Society of Japan | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Biological and Pharmaceutical Bulletin, 37(1), pp.137-144; 2014 | |||||
