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Thrombospondin-1 Is a Putative Target Gene of Runx2 and Runx3
http://hdl.handle.net/10069/33643
http://hdl.handle.net/10069/3364308e403d6-840b-4781-8f55-0926314dbdb3
名前 / ファイル | ライセンス | アクション |
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IJMS14_14321.pdf (879.5 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2013-09-12 | |||||
タイトル | ||||||
タイトル | Thrombospondin-1 Is a Putative Target Gene of Runx2 and Runx3 | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Angiogenesis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | B16-F10 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | C3H10T1/2 stem cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cancer | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Melanoma | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Metastasis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Osteoblast | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Runx2 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Runx3 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | TSP-1 | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Shi, Xiuming
× Shi, Xiuming× Deepak, Vishwa× Wang, Linghui× Ba, Xueqing× Komori, Toshihisa× Zeng, Xianlu× Liu, Wenguang |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Thrombospondin-1 (TSP-1), a matricellular protein widely acclaimed to be involved in the inhibition of angiogenesis and tumorigenesis, is synthesized and secreted by many cell types, including osteoblast and cancer cells. TSP-1 is highly upregulated during early stage of osteogenesis, whereas it inhibits terminal osteoblast differentiation. Expression of TSP-1 is downregulated in cancer cells, and its ectopic expression has been shown to restrain tumor growth. Transcriptional regulation of TSP-1 in osteogenesis and cancer is poorly understood; this prompted us to study its regulation by the two key regulators of the aforementioned processes: Runx2 and Runx3. Through a PCR-based cDNA subtraction technique, we identified and cloned a cDNA fragment for mouse TSP-1, whose expression was dramatically upregulated in response to Runx2 expression in mesenchymal stem cells. Moreover, TSP-1 expression was considerably reduced in the lung of Runx2 knockout mouse. On the other hand, TSP-1 gene expression drastically increased at both the transcriptional and translational levels in response to Runx3 expression in B16-F10 melanoma cells. In line with this, Runx2 and Runx3 bound to the TSP-1 promoter and stimulated its activity. Hence, these results provide first line of evidence that TSP-1 is a transcriptional target gene of Runx2 and Runx3. | |||||
書誌情報 |
International Journal of Molecular Sciences 巻 14, 号 7, p. 14321-14332, 発行日 2013-07-10 |
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出版者 | ||||||
出版者 | Multidisciplinary Digital Publishing Institute (MDPI) | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 14220067 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3390/ijms140714321 | |||||
権利 | ||||||
権利情報 | © 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | International Journal of Molecular Sciences, 14(7), pp.14321-14332; 2013 |