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A novel animal model for in vivo study of liver cancer metastasis
http://hdl.handle.net/10069/30143
http://hdl.handle.net/10069/30143eb15d416-07c5-4497-89d3-9ab7ca416064
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
WJG18_3875.pdf (899.2 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2012-12-27 | |||||
| タイトル | ||||||
| タイトル | A novel animal model for in vivo study of liver cancer metastasis | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Alpha-fetoprotein-producing gastric cancer cells | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Liver cancer metastasis | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Mouse with humanized liver | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Urokinase-type plasminogen activator/severe combined immunodeficient mouse | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Fujiwara, Shinsuke
× Fujiwara, Shinsuke× Fujioka, Hikaru× Tateno, Chise× Taniguchi, Ken× Ito, Masahiro× Ohishi, Hiroshi× Utoh, Rie× Ishibashi, Hiromi× Kanematsu, Takashi× Yoshizato, Katsutoshi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | AIM: To establish an animal model with human hepatocyte- repopulated liver for the study of liver cancer metastasis. METHODS: Cell transplantation into mouse livers was conducted using alpha-fetoprotein (AFP)-producing human gastric cancer cells (h-GCCs) and h-hepatocytes as donor cells in a transgenic mouse line expressing urokinase-type plasminogen activator (uPA) driven by the albumin enhancer/promoter crossed with a severe combined immunodeficient (SCID) mouse line (uPA/ SCID mice). Host mice were divided into two groups (A and B). Group A mice were transplanted with h-GCCs alone, and group B mice were transplanted with h-GCCs and h-hepatocytes together. The replacement index (RI), which is the ratio of transplanted h-GCCs and h-hepatocytes that occupy the examined area of a histological section, was estimated by measuring h-AFP and h-albumin concentrations in sera, respectively, as well as by immunohistochemical analyses of h-AFP and human cytokeratin 18 in histological sections. RESULTS: The h-GCCs successfully engrafted, repopulated, and colonized the livers of mice in group A (RI = 22.0% ± 2.6%). These mice had moderately differentiated adenocarcinomatous lesions with disrupted glandular structures, which is a characteristics feature of gastric cancers. The serum h-AFP level reached 211.0 ± 142.2 g/mL (range, 7.1-324.2 g/mL). In group B mice, the h-GCCs and h-hepatocytes independently engrafted, repopulated the host liver, and developed colonies (RI = 12.0% ± 6.8% and 66.0% ± 12.3%, respectively). h-GCC colonies also showed typical adenocarcinomatous glandular structures around the h-hepatocyte- colonies. These mice survived for the full 56 day-study and did not exhibit any metastasis of h-GCCs in the extrahepatic regions during the observational period. The mice with an h-hepatocyte-repopulated liver possessed metastasized h-GCCs and therefore could be a useful humanized liver animal model for studying liver cancer metastasis in vivo. CONCLUSION: A novel animal model of human liver cancer metastasis was established using the uPA/SCID mouse line. This model could be useful for in vivo testing of anti-cancer drugs and for studying the mechanisms of human liver cancer metastasis. | |||||
| 書誌情報 |
World Journal of Gastroenterology 巻 18, 号 29, p. 3875-3882, 発行日 2012-08-07 |
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| 出版者 | ||||||
| 出版者 | WJG Press | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 10079327 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.3748/wjg.v18.i29.3875 | |||||
| 権利 | ||||||
| 権利情報 | © 1995-2012 Baishideng Publishing Group Co., Limited. Articles published by this Open-Access journal are distributed under the terms of the Creative Commons Attribution Non-commercial License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited, the use is non commercial and is otherwise in compliance with the license. | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | World Journal of Gastroenterology, 18(29), pp.3875-3882; 2012 | |||||
