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Effects of eluted components from 4-META/MMA-TBB adhesive resin sealer on osteoblastic cell proliferation
http://hdl.handle.net/10069/29381
http://hdl.handle.net/10069/2938171feea76-3df5-4899-afa0-278539631471
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
JDS7_94.pdf (407.9 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2012-10-04 | |||||
| タイトル | ||||||
| タイトル | Effects of eluted components from 4-META/MMA-TBB adhesive resin sealer on osteoblastic cell proliferation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | adhesive resin sealer | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | boron concentration | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | MAPK expression | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | MTT assay | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | osteoblastic cell proliferation | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Kawasaki, Aya
× Kawasaki, Aya× Hayashi, Yoshihiko× Yanagiguchi, Kajiro× Yamada, Shizuka× Syudo, Minoru× Igawa, Kazunari× Ikeda, Takeshi× Kubo, Shisei× Fujiwara, Mamoru |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Background/purpose: Adhesive resin sealer systems are applied to seal root-canal systems more effectively through the formation of a resin impregnation layer. The purpose of this study was to investigate the effects of eluted components of the adhesive, Super-Bond sealer (SBS), on the proliferation of osteoblastic cells in vitro. Materials and methods: The standard powder:liquid ratio according to the manufacturer's instructions was used to produce a cylindrical block of SBS (5 mm in diameter, 10 mm long) for this elution study. The resin block was placed on a 100-mm culture dish. Osteoblastic cells were seeded at a density of 4 × 10 6 in α-minimum essential medium containing 10% fetal bovine serum and cultured in a humidified incubator. After 3 days of culture with or without SBS, cells were retrieved and lysed according to the manufacturer's instructions. The cellular events induced by the eluted components from SBS were analyzed using an antibody assay for mitogen-activated protein kinases (MAPKs) and a 3-(4,5-dimethylthyazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Furthermore, the concentration of boron, a component of the catalyst, tri-n-butyl borane (TBB), was analyzed using inductively coupled plasma optical emission spectrometry. Results: Expression of MAPKs increased after SBS application. The MTT assay indicated that TBB, one of the components of SBS, accelerated the proliferation of osteoblastic cells. Values of boron were 1.66 ± 0.37 and 1.74 ± 0.30 ppm in cells cultured with and without FBS, respectively. Conclusions: The eluted components from SBS can increase the expression of some MAPKs related to osteoblastic cell proliferation and differentiation in vitro. Both the elution experiment and treatment of cell culture with SBS components indicated that the boron originating from TBB is likely to be responsible for activation of the proliferation of osteoblastic cells. | |||||
| 書誌情報 |
Journal of Dental Sciences 巻 7, 号 2, p. 94-98, 発行日 2012-06 |
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| 出版者 | ||||||
| 出版者 | Association for Dental Sciences of the Republic of China | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 19917902 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.jds.2012.03.002 | |||||
| 権利 | ||||||
| 権利情報 | © 2012, Association for Dental Sciences of the Republic of China. Published by Elsevier Taiwan LLC. All rights reserved. | |||||
| 権利 | ||||||
| 権利情報 | NOTICE: this is the author’s version of a work that was accepted for publication in Journal of Dental Sciences. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Dental Sciences, 7, 2(2012) | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Journal of Dental Sciences, 7(2), pp.94-98; 2012 | |||||
