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Reduced Light Response of Neuronal Firing Activity in the Suprachiasmatic Nucleus and Optic Nerve of Cryptochrome-Deficient Mice
http://hdl.handle.net/10069/27319
http://hdl.handle.net/10069/273195fff475d-b589-4f0b-b55a-73c5db94d874
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
PLoS6_28726.pdf (314.2 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2012-01-30 | |||||
| タイトル | ||||||
| タイトル | Reduced Light Response of Neuronal Firing Activity in the Suprachiasmatic Nucleus and Optic Nerve of Cryptochrome-Deficient Mice | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Nakamura, Takahiro J.
× Nakamura, Takahiro J.× Ebihara, Shizufumi× Shinohara, Kazuyuki |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Abstract \nTo examine roles of the Cryptochromes (Cry1 and Cry2) in mammalian circadian photoreception, we recorded single-unit neuronal firing activity in the suprachiasmatic nucleus (SCN), a primary circadian oscillator, and optic nerve fibers in vivo after retinal illumination in anesthetized Cry1 and Cry2 double-knockout (Cry-deficient) mice. In wild-type mice, most SCN neurons increased their firing frequency in response to retinal illumination at night, whereas only 17% of SCN neurons responded during the daytime. However, 40% of SCN neurons responded to light during the daytime, and 31% of SCN neurons responded at night in Cry-deficient mice. The magnitude of the photic response in SCN neurons at night was significantly lower (1.3-fold of spontaneous firing) in Cry-deficient mice than in wild-type mice (4.0-fold of spontaneous firing). In the optic nerve near the SCN, no difference in the proportion of light-responsive fibers was observed between daytime and nighttime in both genotypes. However, the response magnitude in the light-activated fibers (ON fibers) was high during the nighttime and low during the daytime in wild-type mice, whereas this day-night difference was not observed in Cry-deficient mice. In addition, we observed day-night differences in the spontaneous firing rates in the SCN in both genotypes and in the fibers of wild-type, but not Cry-deficient mice. We conclude that the low photo response in the SCN of Cry-deficient mice is caused by a circadian gating defect in the retina, suggesting that Cryptochromes are required for appropriate temporal photoreception in mammals. |
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| 書誌情報 |
PLoS ONE 巻 6, 号 12, p. e28726, 発行日 2011-12-21 |
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| 出版者 | ||||||
| 出版者 | Public Library of Science | |||||
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| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 19326203 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1371/journal.pone.0028726 | |||||
| 権利 | ||||||
| 権利情報 | © 2011 Nakamura et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | PLoS ONE, 6(12), pp.e28726; 2011 | |||||
