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アイテム
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Investigation of Intracellular Delivery of NuBCP-9 by Conjugation with Oligoarginines Peptides in MDA-MB-231 Cells
http://hdl.handle.net/10069/38602
http://hdl.handle.net/10069/3860239e8aefa-13d0-474c-b34d-c3c5afa49186
名前 / ファイル | ライセンス | アクション |
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BPBul41_1448.pdf (3.3 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-09-27 | |||||
タイトル | ||||||
タイトル | Investigation of Intracellular Delivery of NuBCP-9 by Conjugation with Oligoarginines Peptides in MDA-MB-231 Cells | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | B-cell lymphoma 2 (Bcl-2) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Endocytosis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ntracellular delivery | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | NuBCP-9 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Oligoarginine | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Wang, Wei
× Wang, Wei× Suga, Tadaharu× Hagimori, Masayori× Kuroda, Naotaka× Fuchigami, Yuki× Kawakami, Shigeru |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Oligoarginines (Rn) are becoming promising tools for the intracellular delivery of biologically active molecules. NuBCP-9, a peptide that induces apoptosis in B-cell lymphoma 2 (Bcl-2)-expressing cancer cells, has been reported to promote the uptake and non-specific cytotoxicity of R8, also called octaarginine. However, it is unknown whether a similar synergistic effect can be seen with other Rn. In this study, we conjugated NuBCP-9 with various Rn (n=8, 10, 12, 14) to investigate and compare their cellular uptake characteristics. In addition, their non-specific cytotoxicity and apoptosis-inducing abilities were evaluated. We found that NuBCP-9 conjugated with Rn enhanced cellular uptake mainly through clathrin-mediated endocytosis and macropinocytosis, and that the uptake pathways were not different from those used by unconjugated Rn. However, the cytotoxicity study showed that NuBCP-9-R12 and NuBCP-9-R14 conjugates enhanced nonspecific cytotoxicity. We found that NuBCP-9-R10 conjugate had the highest uptake efficiency and induced correspondingly high levels of apoptosis, while resulting in a tolerable degree of non-specific toxicity. | |||||
書誌情報 |
Biological and Pharmaceutical Bulletin 巻 41, 号 9, p. 1448-1455, 発行日 2018-09-01 |
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出版者 | ||||||
出版者 | 日本薬学会 | |||||
出版者別言語 | ||||||
Pharmaceutical Society of Japan | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09186158 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13475215 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1248/bpb.b18-00335 | |||||
権利 | ||||||
権利情報 | c 2018 The Pharmaceutical Society of Japan. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Biological and Pharmaceutical Bulletin, 41(9), pp.1448-1455; 2018 |