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Development of a simple and convenient feeding device to infect Aedes aegypti mosquitoes with Brugia pahangi microfilariae derived from the peritoneal cavity of Mongolian jirds
http://hdl.handle.net/10069/23995
http://hdl.handle.net/10069/23995162cd32b-f5d3-43b2-8303-dbe3e7059f4c
名前 / ファイル | ライセンス | アクション |
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TMH38_97.pdf (140.9 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-09-21 | |||||
タイトル | ||||||
タイトル | Development of a simple and convenient feeding device to infect Aedes aegypti mosquitoes with Brugia pahangi microfilariae derived from the peritoneal cavity of Mongolian jirds | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Mitsui, Yoshinori
× Mitsui, Yoshinori× Miura, Mitsumasa |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | It has become difficult in recent years to conduct the direct feeding of mosquitoes on animals because of ethical considerations related to animal experimentation. Thus, the artificial feeding of mosquitoes on blood meals is an important technique in studies on the oral infection of mosquitoes to agents. Since Rutledge et al. (1964) devised the artificial membrane-feeding technique, several artificial membrane-feeding methods have been developed to increase the feeding rates of mosquitoes on blood meals. The purpose of the present study is to develop a simple and convenient device for the artificial feeding of mosquitoes. We designed a device using Kimwipe®, a coverglass, the lid of a plastic dish and a 50 ml Erlenmeyer flask. The efficacy was assessed by the infection rate of mosquitoes to Brugia pahangi microfilariae (MF) derived from the peritoneal cavity of Mongolian jirds. Immediately after the feeding of mosquitoes on MF by the new device, the MF infection rate of mosquitoes was 50 - 81%. On day 14 post-feeding, 51 - 94% of mosquitoes harbored third-stage infective larvae. The components needed to construct the device for artificial feeding of mosquitoes are generally available in laboratories. Furthermore, no elaborate modification of materials is necessary in making the feeding device. Therefore, this simple and convenient artificial feeding device promises to be applicable for experimental infection of mosquitoes not only with B. pahangi MF but also with other agents such as malaria and viruses. | |||||
書誌情報 |
Tropical Medicine and Health 巻 38, 号 3, p. 97-101, 発行日 2010-09 |
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出版者 | ||||||
出版者 | Japanese Society of Tropical Medicine | |||||
出版者別言語 | ||||||
日本熱帯医学会 | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13488945 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13494147 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11912846 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.2149/tmh.2010-07 | |||||
権利 | ||||||
権利情報 | Copyright (c) 2010 by The Japanese Society of Tropical Medicine | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Tropical Medicine and Health, 38(3), pp.97-101; 2010 |