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Direct binding of gangliosides to Helicobacter pylori vacuolating cytotoxin (VacA) neutralizes its toxin activity.
http://hdl.handle.net/10069/25393
http://hdl.handle.net/10069/253938d585794-b5cd-4b84-9f8d-efba7b62cebe
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Gly20_668.pdf (2.1 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2011-07-25 | |||||
| タイトル | ||||||
| タイトル | Direct binding of gangliosides to Helicobacter pylori vacuolating cytotoxin (VacA) neutralizes its toxin activity. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Fluorescence correlation spectroscopy | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Ganglioside | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | GM1 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Helicobacter pylori | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Helicobacter pylori | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Wada, Akihiro
× Wada, Akihiro× Hasegawa, Makoto× Wong, Pooi-Fong× Shirai, Emi× Shirai, Nobuaki× Tan, Li-Jing× Llanes, Rafael× Hojo, Hironobu× Yamasaki, Eiki× Ichinose, Akitoyo× Ichinose, Yoshio× Senba, Masachika |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Gangliosides are target receptors for bacterial entry, yet those present in human milk exhibit a protective role against bacterial infection. Here, we show that treatment with ganglioside mixture at a concentration of 100 microg/mL resulted in significant inhibition of the vacuole formation activity of Helicobacter pylori vacuolating cytotoxin (VacA) in gastric epithelial cancer AZ-521 cells. All gangliosides (GM1, GM2, GM3, GD1a, GD1b, GD3 and GT1b) examined showed good neutralizing capacity against VacA. A pull-down assay was performed using lyso-GM1 coupled to Sepharose as the tagged polysaccharide polymer to capture VacA from H. pylori culture supernatant. GM1-VacA complexes were successfully precipitated, suggesting that GM1 binds directly to VacA. The hydrodynamic binding of lyso-GM1 and VacA measured by fluorescence correlation spectroscopy had a K(d) value of 190 nM. VacA also bound to lyso-GM1 at pH 2 corresponding to the physiological pH of human stomach. Collectively, these results showed that direct binding of H. pylori VacA to free gangliosides neutralizes the toxin activity of VacA. These findings offer an alternative insight into the role of gangliosides in VacA toxicity and the pathogenesis of H. pylori. | |||||
| 書誌情報 |
Glycobiology 巻 20, 号 6, p. 668-678, 発行日 2010-06 |
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| 出版者 | ||||||
| 出版者 | Oxford University Press | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 09596658 | |||||
| EISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 14602423 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10826501 | |||||
| PubMed番号 | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 20118071 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1093/glycob/cwq014 | |||||
| 権利 | ||||||
| 権利情報 | © The Author 2010. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org. | |||||
| 権利 | ||||||
| 権利情報 | This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Glycobiology following peer review. The definitive publisher-authenticated version Glycobiology, 20(6), pp.668-678; 2010 is available online at: http://dx.doi.org/10.1093/glycob/cwq014. | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Glycobiology, 20(6), pp.668-678; 2010 | |||||
