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Comparison of enzyme-linked immunosorbent assay systems using rift valley fever virus nucleocapsid protein and inactivated virus as antigens
http://hdl.handle.net/10069/38733
http://hdl.handle.net/10069/3873315dcf815-1500-41fd-ab62-ff6ba76c74f4
名前 / ファイル | ライセンス | アクション |
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VirJour15_178.pdf (789.7 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-12-18 | |||||
タイトル | ||||||
タイトル | Comparison of enzyme-linked immunosorbent assay systems using rift valley fever virus nucleocapsid protein and inactivated virus as antigens | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Expression | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | IgG sandwich ELISA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | IgM capture ELISA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Nucleocapsid protein | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | RVFV | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Yu, Fuxun
× Yu, Fuxun× Adungo, Ferdinard× Konongoi, Samson Limbaso× Inoue, Shingo× Sang, Rosemary× Ashur, Salame× Kwallah, Allan ole× Uchida, Leo× Buerano, Corazon C× Mwau, Matilu× Zha, Yan× Nie, Yingjie× Morita, Kouichi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | BACKGROUND: Rift Valley Fever(RVF)is a mosquito-borne viral zoonosis.To detect RVF virus(RVFV)infection,indirect immunoglobulin G(IgG)and immunoglobulin M(IgM)enzyme linked immunosorbent assays(ELISAs)which utilize recombinant RVFV nucleocapsid(RVFV-N)protein as assay antigen, have reportedly been used, however, there is still a need to develop more sensitive and specific methods of detection. METHODS: RVFV-N protein was expressed in Escherichia coli (E. coli) and purified by histidine-tag based affinity chromatography. This recombinant RVFV-N (rRVFV-N) protein was then used as antigen to develop an IgG sandwich ELISA and IgM capture ELISAs for human sera. Ninety six serum samples collected from healthy volunteers during the RVF surveillance programme in Kenya in 2013, and 93 serum samples collected from RVF-suspected patients during the 2006-2007 RVF outbreak in Kenya were used respectively, to evaluate the newly established rRVFV-N protein-based IgG sandwich ELISA and IgM capture ELISA systems in comparison with the inactivated virus-based ELISA systems. RESULTS: rRVFV-N protein-based-IgG sandwich ELISA and IgM capture ELISA for human sera were established.Both the new ELISA systems were in 100% concordance with the inactivated virus-based ELISA systems, with a sensitivity and specificity of 100%. CONCLUSIONS: Recombinant RVFV-N is a safe and affordable antigen for RVF diagnosis.Our rRVFV-N-based ELISA systems are safe and reliable tools for diagnosis of RVFV infection in humans and especially useful in large-scale epidemiological investigation and for application in developing countries. | |||||
書誌情報 |
Virology Journal 巻 15, 号 1, p. 178, 発行日 2018-11-22 |
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出版者 | ||||||
出版者 | Bio Med Central | |||||
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収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1743422X | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1186/s12985-018-1071-y | |||||
権利 | ||||||
権利情報 | cThe Author(s). 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Virology Journal, 15(1), 178; 2018 |