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Molecular characterization on the genome structure of hemolysin toxin isoforms isolated from sea anemone Actineria villosa and Phyllodiscus semoni.
http://hdl.handle.net/10069/24835
http://hdl.handle.net/10069/2483500e874e3-2fba-4054-b14c-1141d80e3b76
名前 / ファイル | ライセンス | アクション |
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Toxicon56_1470.pdf (495.5 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2011-03-31 | |||||
タイトル | ||||||
タイトル | Molecular characterization on the genome structure of hemolysin toxin isoforms isolated from sea anemone Actineria villosa and Phyllodiscus semoni. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Actineria villosa | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Actinoporin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Hemolysin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Intron | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Isoform | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Multigene | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Phyllodiscus semoni | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Sea anemone | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Uechi, Gen-Ichiro
× Uechi, Gen-Ichiro× Toma, Hiromu× Arakawa, Takeshi× Sato, Yoshiya |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We recently identified the existence of new isoforms of Avt-I (from sea anemone Actineria villosa) and Pstx20 (from sea anemone Phyllodiscus semoni) hemolytic toxins, and named them Avt-II and Pst-I. Avt-II and Pst-I differ in length by 14 and 7 bp, respectively, as compared to their corresponding isoform genes. Both newly found isoform genes have the coding regions with the identical length of 1033 bp. The restriction fragment length polymorphism analysis with endonuclease HphI was able to clearly distinguish between the two Avt isoforms, but not Pstx isoforms, and based on the densitometric analysis of DNA bands, it indicated that relative expression levels of Avt-I and Avt-II genes were 18.3% and 81.7%, respectively. PCR amplification of the two Avt isoform genes using the genomic DNA as template indicated the existence of two introns within each toxin isoform gene. The first intron with the identical 242 bp in length for both Avt isoform was found within the 5'-untranslated region, and the second intron with lengths of 654 bp and 661 bp in Avt-I and Avt-II isoforms, respectively, was found within the signal sequence coding region. This is for the first time to identify the existence of introns within hemolysin genes of sea anemone. Having several unique characteristics that have identified only for a new member of actinoporin family of A. villosa and P. semoni, e.g., strong toxicity and genes with introns, it is plausible to speculate that these toxins have a unique genetic evolutionary linage differed from that for other sea anemone hemolytic toxins. | |||||
書誌情報 |
Toxicon : official journal of the International Society on Toxinology 巻 56, 号 8, p. 1470-1476, 発行日 2010-12 |
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出版者 | ||||||
出版者 | Elsevier Ltd. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00410101 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 18793150 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00864479 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 20837039 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.toxicon.2010.09.002 | |||||
権利 | ||||||
権利情報 | Copyright © 2010 Published by Elsevier Ltd. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Toxicon, 56(8), pp.1470-1476; 2010 |