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Novel Babesia bovis exported proteins that modify properties of infected red blood cells
http://hdl.handle.net/10069/40378
http://hdl.handle.net/10069/4037888e9a023-8517-4335-90a2-a52c3cb6f7e3
名前 / ファイル | ライセンス | アクション |
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PLoSPat16_1008917.pdf (2.9 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-11-05 | |||||
タイトル | ||||||
タイトル | Novel Babesia bovis exported proteins that modify properties of infected red blood cells | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Hakimi, Hassan
× Hakimi, Hassan× Templeton, Thomas J.× Sakaguchi, Miako× Yamagishi, Junya× Miyazaki, Shinya× Yahata, Kazuhide× Uchihashi, Takayuki× Kawazu, Shin-ichiro× Kaneko, Osamu× Asada, Masahito |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Babesia bovis causes a pathogenic form of babesiosis in cattle. Following invasion of red blood cells (RBCs) the parasite extensively modifies host cell structural and mechanical properties via the export of numerous proteins. Despite their crucial role in virulence and pathogenesis, such proteins have not been comprehensively characterized in B. bovis. Here we describe the surface biotinylation of infected RBCs (iRBCs), followed by proteomic analysis. We describe a multigene family (mtm) that encodes predicted multi-transmembrane integral membrane proteins which are exported and expressed on the surface of iRBCs. One mtm gene was downregulated in blasticidin-S (BS) resistant parasites, suggesting an association with BS uptake. Induced knockdown of a novel exported protein encoded by BBOV-III004280, named VESA export-associated protein (BbVEAP), resulted in a decreased growth rate, reduced RBC surface ridge numbers, mis-localized VESA1, and abrogated cytoadhesion to endothelial cells,suggesting that BbVEAP is a novel virulence factor for B. bovis. |
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書誌情報 |
PLOS Pathogens 巻 16, 号 10, p. e1008917, 発行日 2020-10-05 |
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出版者 | ||||||
出版者 | Public Library of Science | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 15537374 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1371/journal.ppat.1008917 | |||||
権利 | ||||||
権利情報 | c 2020 Hakimi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License,which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | PLoS Pathogens, 16(10), e1008917; 2020 |