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Structural characterization of Tn916-like element in Streptococcus parauberis serotype II strains isolated from diseased Japanese flounder.
http://hdl.handle.net/10069/23259
http://hdl.handle.net/10069/2325917a89ddb-a13b-4e33-913d-afa677f0ae7b
名前 / ファイル | ライセンス | アクション |
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LAM48_770.pdf (430.5 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-07-01 | |||||
タイトル | ||||||
タイトル | Structural characterization of Tn916-like element in Streptococcus parauberis serotype II strains isolated from diseased Japanese flounder. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Japan | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Japanese flounder | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | serotype | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Streptococcus parauberis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | tetracycline resistance | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Tn916 | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Meng, Fei
× Meng, Fei× Kanai, Kinya× Yoshikoshi, Kazuma |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | AIMS: To screen for the existence and determine the structure of Tn916-like element in Streptococcus parauberis serotype II strains isolated from cultured Japanese flounder in western Japan. METHODS AND RESULTS: In this study, the structure of Tn916-like element and the flanking regions were characterized by polymerase chain reaction (PCR) and inverse PCR, followed by cloning and sequencing. The Tn916-like element is 18 031 bp in length and composed of 22 ORFs. Southern blot hybridization analysis showed that the HincII-digested internal structures of Tn916-like elements yielded two patterns among S. parauberis serotype II strains. The flanking sequences were identical with the corresponding region of S. parauberis serotype I strain except for the addition of 6-bp coupling sequence (ATCATA) being adjacent to the upstream of the element. CONCLUSION: The Tn916-like element exhibited high homology (more than 99%) with Tn916 observed in other streptococci and enterococci and was integrated in the same site of chromosome for all of the tested S. parauberis serotype II strains. SIGNIFICANCE AND IMPACT OF THE STUDY: The results indicate that the Tn916-like element encoding tet(M) gene is present in all of the tested S. parauberis serotype II strains, which are disseminated in the flounder-culturing areas in western Japan. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | The definitive version is available at www.blackwell-synergy.com | |||||
書誌情報 |
Letters in applied microbiology 巻 48, 号 6, p. 770-776, 発行日 2009-06 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 02668254 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1472765X | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10462245 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 19344360 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1111/j.1472-765X.2009.02609.x | |||||
権利 | ||||||
権利情報 | © 2009 The Society for Applied Microbiology. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Letters in applied microbiology, 48(6), pp.770-776; 2009 |