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Diagnosis of visceral leishmaniasis by polymerase chain reaction of DNA extracted from Giemsa's solution-stained slides.
http://hdl.handle.net/10069/25632
http://hdl.handle.net/10069/25632ef168825-069f-473b-902f-e0f1a09fc85a
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ParRes107_727.pdf (276.9 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-09-01 | |||||
| タイトル | ||||||
| タイトル | Diagnosis of visceral leishmaniasis by polymerase chain reaction of DNA extracted from Giemsa's solution-stained slides. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Visceral Leishmaniasis | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | PCR | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | bone marrow | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Nepal | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Pandey, Kishor
× Pandey, Kishor× Pandey, Basu Dev× Mallik, Arun Kumar× Kaneko, Osamu× Uemura, Haruki× Kanbara, Hiroji× Yanagi, Tetsuo× Hirayama, Kenji |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Visceral leishmaniasis (VL) is caused by the protozoan parasite Leishmania donovani and is a potentially fatal disease in endemic areas of the world. Nepal is an endemic area in which VL causes major public health problems in the lowland areas of the southeast regions. The aim of the present study was to evaluate the sensitivity of polymerase chain reaction (PCR) amplification for the detection of Leishmania DNA from Giemsa's solution-stained bone marrow slides. Bone marrow samples were aspirated from a total of 115 VL suspected patients and used to prepare smears on glass slides and for the initiation of in vitro culture. Bone marrow slides were used for microscopic observation, DNA extraction, and subsequent PCR amplification. PCR analysis showed that all the positive samples were of Leishmania parasites. The PCR assay also showed a higher sensitivity (69%) than microscopic examination (57%) and culture (21%). In addition, PCR was able to detect VL in 12% of samples which were negative by microscopy. PCR of DNA extracted from Giemsa's solution-stained bone marrow slides is a suitable tool for confirming diagnosis in patients with VL and may also be useful in the diagnosis of difficult cases. Bone marrow smears are easily stored and can be easily sent to research centers where PCR is available. This makes PCR a good option for diagnosis in the field. | |||||
| 書誌情報 |
Parasitology Research 巻 107, 号 3, p. 727-730, 発行日 2010-08 |
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| 出版者 | ||||||
| 出版者 | Springer | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 09320113 | |||||
| EISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 14321955 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10682117 | |||||
| PubMed番号 | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 20499093 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1007/s00436-010-1920-0 | |||||
| 権利 | ||||||
| 権利情報 | © Springer-Verlag 2010 | |||||
| 権利 | ||||||
| 権利情報 | The original publication is available at www.springerlink.com | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Parasitology Research, 107(3), pp.727-730; 2010 | |||||
