WEKO3
アイテム
{"_buckets": {"deposit": "b6630112-1b3f-423b-92ce-f6c675fea15a"}, "_deposit": {"created_by": 2, "id": "15365", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "15365"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00015365", "sets": ["74"]}, "author_link": ["56876", "56871", "56875", "56873", "56878", "56874", "56872", "56877"], "item_2_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2010-08-15", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "4", "bibliographicPageEnd": "547", "bibliographicPageStart": "540", "bibliographicVolumeNumber": "80", "bibliographic_titles": [{"bibliographic_title": "Biochemical pharmacology"}]}]}, "item_2_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Studies suggest that pre-administration of docetaxel (DOC) in adriamycin (ADR)-DOC combination anticancer therapy results in stronger antitumor effects and fewer ADR-induced cardiotoxic deaths in mouse model, yet no mechanism explaining this effect has been established. The aim of this study was to identify cellular processes in mouse heart tissue affected by different ADR/DOC dosing protocols using a toxicoproteomic approach. We applied fluorogenic derivatization-liquid chromatography-tandem mass spectrometry (FD-LC-MS/MS) - which consists of fluorogenic derivatization, separation and fluorescence detection by LC, and identification by LC-tandem mass spectrometry - to the proteomic analysis of heart tissue from control, intermittent-dosing (DOC-ADR), and simultaneous-dosing (ADR\u0026DOC) groups. In DOC-ADR group, ADR was administered 12h after DOC injection; in ADR\u0026DOC group, both drugs were administered simultaneously; in control group, saline was administered at the same timing as ADR injection of other groups. Heart samples were isolated from all mice 1 week after the treatment. The highly reproducible and sensitive method (FD-LC-MS/MS) identified nine proteins that were differentially expressed in heart tissue of control and the two treatment groups; seven of these nine proteins participate in cellular energy production pathways, including glycolysis, the tricarboxylic acid cycle, and the mitochondrial electron transport chain. Significantly higher expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was observed in the DOC-ADR group, the group with the fewer cardiotoxic deaths, than in the ADR\u0026DOC group. Therefore, GAPDH may have potential as a drug target for protective intervention and a biomarker for evaluation of the cardioprotective effects in pre-clinical studies.", "subitem_description_type": "Abstract"}]}, "item_2_description_63": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Biochemical pharmacology, 80(4), pp.540-547; 2010", "subitem_description_type": "Other"}]}, "item_2_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier Inc."}]}, "item_2_relation_11": {"attribute_name": "PubMed番号", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "20457137", "subitem_relation_type_select": "PMID"}}]}, "item_2_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1016/j.bcp.2010.04.037", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "Copyright © 2010 Elsevier Inc. All rights reserved."}]}, "item_2_source_id_10": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00564486", "subitem_source_identifier_type": "NCID"}]}, "item_2_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00062952", "subitem_source_identifier_type": "ISSN"}]}, "item_2_source_id_8": {"attribute_name": "EISSN", "attribute_value_mlt": [{"subitem_source_identifier": "18732968", "subitem_source_identifier_type": "ISSN"}]}, "item_2_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Ohyama, Kaname"}], "nameIdentifiers": [{"nameIdentifier": "56871", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tomonari, Mari"}], "nameIdentifiers": [{"nameIdentifier": "56872", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ichibangase, Tomoko"}], "nameIdentifiers": [{"nameIdentifier": "56873", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "To, Hideto"}], "nameIdentifiers": [{"nameIdentifier": "56874", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kishikawa, Naoya"}], "nameIdentifiers": [{"nameIdentifier": "56875", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakashima, Kenichiro"}], "nameIdentifiers": [{"nameIdentifier": "56876", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Imai, Kazuhiro"}], "nameIdentifiers": [{"nameIdentifier": "56877", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kuroda, Naotaka"}], "nameIdentifiers": [{"nameIdentifier": "56878", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-23"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "BioPha80_540.pdf", "filesize": [{"value": "996.9 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 996900.0, "url": {"label": "BioPha80_540.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/15365/files/BioPha80_540.pdf"}, "version_id": "1ab61648-a196-4150-a8e5-6654f9aefe09"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Adriamycin-induced cardiotoxicity", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Docetaxel pre-administration", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Fluorogenic derivatization-liquid chromatography-tandem mass spectrometry", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Toxicoproteomics", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "A toxicoproteomic study on cardioprotective effects of pre-administration of docetaxel in a mouse model of adriamycin-induced cardiotoxicity.", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "A toxicoproteomic study on cardioprotective effects of pre-administration of docetaxel in a mouse model of adriamycin-induced cardiotoxicity."}]}, "item_type_id": "2", "owner": "2", "path": ["74"], "permalink_uri": "http://hdl.handle.net/10069/23250", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-07-01"}, "publish_date": "2010-07-01", "publish_status": "0", "recid": "15365", "relation": {}, "relation_version_is_last": true, "title": ["A toxicoproteomic study on cardioprotective effects of pre-administration of docetaxel in a mouse model of adriamycin-induced cardiotoxicity."], "weko_shared_id": 2}
A toxicoproteomic study on cardioprotective effects of pre-administration of docetaxel in a mouse model of adriamycin-induced cardiotoxicity.
http://hdl.handle.net/10069/23250
http://hdl.handle.net/10069/2325058e9f8ef-d936-4f0e-8c14-0c99a16a7888
名前 / ファイル | ライセンス | アクション |
---|---|---|
BioPha80_540.pdf (996.9 kB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2010-07-01 | |||||
タイトル | ||||||
タイトル | A toxicoproteomic study on cardioprotective effects of pre-administration of docetaxel in a mouse model of adriamycin-induced cardiotoxicity. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Adriamycin-induced cardiotoxicity | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Docetaxel pre-administration | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Fluorogenic derivatization-liquid chromatography-tandem mass spectrometry | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Toxicoproteomics | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Ohyama, Kaname
× Ohyama, Kaname× Tomonari, Mari× Ichibangase, Tomoko× To, Hideto× Kishikawa, Naoya× Nakashima, Kenichiro× Imai, Kazuhiro× Kuroda, Naotaka |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Studies suggest that pre-administration of docetaxel (DOC) in adriamycin (ADR)-DOC combination anticancer therapy results in stronger antitumor effects and fewer ADR-induced cardiotoxic deaths in mouse model, yet no mechanism explaining this effect has been established. The aim of this study was to identify cellular processes in mouse heart tissue affected by different ADR/DOC dosing protocols using a toxicoproteomic approach. We applied fluorogenic derivatization-liquid chromatography-tandem mass spectrometry (FD-LC-MS/MS) - which consists of fluorogenic derivatization, separation and fluorescence detection by LC, and identification by LC-tandem mass spectrometry - to the proteomic analysis of heart tissue from control, intermittent-dosing (DOC-ADR), and simultaneous-dosing (ADR&DOC) groups. In DOC-ADR group, ADR was administered 12h after DOC injection; in ADR&DOC group, both drugs were administered simultaneously; in control group, saline was administered at the same timing as ADR injection of other groups. Heart samples were isolated from all mice 1 week after the treatment. The highly reproducible and sensitive method (FD-LC-MS/MS) identified nine proteins that were differentially expressed in heart tissue of control and the two treatment groups; seven of these nine proteins participate in cellular energy production pathways, including glycolysis, the tricarboxylic acid cycle, and the mitochondrial electron transport chain. Significantly higher expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was observed in the DOC-ADR group, the group with the fewer cardiotoxic deaths, than in the ADR&DOC group. Therefore, GAPDH may have potential as a drug target for protective intervention and a biomarker for evaluation of the cardioprotective effects in pre-clinical studies. | |||||
書誌情報 |
Biochemical pharmacology 巻 80, 号 4, p. 540-547, 発行日 2010-08-15 |
|||||
出版者 | ||||||
出版者 | Elsevier Inc. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00062952 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 18732968 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00564486 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 20457137 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.bcp.2010.04.037 | |||||
権利 | ||||||
権利情報 | Copyright © 2010 Elsevier Inc. All rights reserved. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Biochemical pharmacology, 80(4), pp.540-547; 2010 |