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Facile detection of proteins on a solid-phase membrane by direct binding of dextran-based luminol?biotin chemiluminescent polymer
http://hdl.handle.net/10069/22005
http://hdl.handle.net/10069/220058102e2dc-a5e4-4304-8413-bc9e3594f880
名前 / ファイル | ライセンス | アクション |
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Talanta79_700.pdf (548.3 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2009-06-23 | |||||
タイトル | ||||||
タイトル | Facile detection of proteins on a solid-phase membrane by direct binding of dextran-based luminol?biotin chemiluminescent polymer | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Chemiluminescence | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Luminol-containing macromolecule | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Microchip | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Protein detection | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Zhang, Huan
× Zhang, Huan× Shibata, Takayuki× Krawczyk, Tomasz× Kabashima, Tsutomu× Lu, Jianzhong× Lee, Myung K.× Kai, Masaaki |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Facile and non-radioactive methods are desired for the sensitive detection and quantification of various proteins. Herein we describe a novel chemiluminescence (CL)-detection method of particular proteins based on direct binding of a dextran-luminol-biotin (DLB) CL polymer to the proteins on a poly(vinylidene difluoride) membrane. Among 32 kinds of the proteins screened, several proteins such as drug-metabolizing enzymes, cytochrome p450 (CYP)1A2, CYP2E1, and CYP3A4 had the ability to bind directly to the DLB polymer. The binding site in the polymer was owing to the framework of the modified dextran, which underwent oxidation and reduction procedures. This interaction might be the comprehensive effect of both electrostatic interaction and steric complementarities. CL intensity of the proteins detected by the polymer could be further enlarged by the mediation of avidin. The proposed CL-imaging method possesses potential as a rapid, facile, inexpensive and selective detection of the proteins. | |||||
書誌情報 |
Talanta 巻 79, 号 3, p. 700-705, 発行日 2009-08 |
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出版者 | ||||||
出版者 | Elsevier B.V. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00399140 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11539538 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.talanta.2009.04.056 | |||||
権利 | ||||||
権利情報 | (c) 2009 Elsevier B.V. All rights reserved. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Talanta, 79(3), pp.700-705; 2009 |